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| Status |
Public on Jan 14, 2020 |
| Title |
Effects of Electronic Cigarette Constituents on the Human Lung: A Pilot Clinical Trial [methylation] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
E-cig use is continuing to increase, particularly among youth never-smokers, and is used by some smokers to quit. The acute and chronic toxicity of e-cig use is unclear generally in the context of increasing reports of inflammatory-type pneumonia in some e-cig users. To assess lung effects of e-cigs without nicotine or flavors, we conducted a pilot study with serial bronchoscopies over 4 weeks in 30 never-smokers, randomized either to a four-week intervention with the use of e-cigs containing only 50% propylene glycol (PG) and 50% vegetable glycerine (VG) or to a no-use control group. Compliance to the e-cig intervention was assessed by participants sending daily puff counts and by urinary propylene glycol (PG). Inflammatory cell counts and cytokines were determined in bronchoalveolar lavage (BAL) fluids. Genome-wide expression, microRNA, and mRNA were determined from bronchial epithelial cells. There were no significant differences in changes of BAL inflammatory cell counts or cytokines between baseline and follow-up, comparing the control and e-cig groups. However, in the intervention but not the control group, change in urinary PG as a marker of e-cig use and inhalation, was significantly correlated with change in cell counts (cell concentrations, macrophages, and lymphocytes) and cytokines (IL-8, IL-13, and TNF-α), although the absolute magnitude of changes was small. There were no significant changes in mRNA or microRNA gene expression. Although limited by study size and duration, this is the first experimental demonstration of an impact of e-cig use on inflammation in the human lung among never-smokers.
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| Overall design |
Bisulphite converted DNA from the 58 samples were hybridised to the Illumina Infinium 850k Human Methylation Beadchips
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| Contributor(s) |
Song M, Weng DY, Shields PG |
| Citation(s) |
31619441 |
| Submission date |
Oct 02, 2019 |
| Last update date |
Jan 14, 2020 |
| Contact name |
Daniel Yui Weng |
| E-mail(s) |
daniel.weng@osumc.edu
|
| Phone |
614-3664068
|
| Organization name |
The Ohio State University
|
| Department |
Comprehensive Cancer Center
|
| Street address |
420 W. 12th Ave. Room 390
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
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| Samples (58)
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| This SubSeries is part of SuperSeries: |
| GSE138351 |
Effects of Electronic Cigarette Constituents on the Human Lung: A Pilot Clinical Trial |
|
| Relations |
| BioProject |
PRJNA575552 |
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