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Series GSE13835 Query DataSets for GSE13835
Status Public on May 15, 2009
Title Smooth muscle cells in atherosclerosis-prone and resistant regions of the aorta of C57Bl/6 mice at age of 4 months
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study compared gene expression in smooth muscle cells (SMCs) in atherosclerosis-prone and atherosclerosis-resistant regions of the aorta of C57Bl/6 mice. In a parallel experiment, both regions were compared in young, plaque-free apolipoprotein E-deficient (apoE-/-) mice. Aortas of 3 male and 3 female C57Bl6 mice were isolated, perfused with triton X-100 to remove endothelial cells and divided in an atherosclerosis-prone region (AA: ascending aorta, aortic arch and proximal 2 mm of thoracic aorta) and an atherosclerosis-resistant region (TA: central thoracic aorta,i.e. 6 mm distal from the proximal 2mm of the thoracic aorta). Microarray analysis (VIB-MAF) of pooled total RNA showed differential expression (>2-fold difference) for 70 genes. Up- or downregulation in the AA was observed for 33 and 37 genes respectively. Differential expression of 3 genes (ATPase, Na+/K+ transporting, beta 1 polypeptide, sarcolipin and homeo box B7) was confirmed using real-time PCR. Twenty five genes showed exclusively differential expression in C57BL6 mice. Only 7 could be linked to specific processes: development (4) and cell growth (3). The other 18 genes were all involved in different processes. Among the 45 genes showing differential expression in C57Bl/6 as well as apoE-/- mice, most were related to development (13), cell growth (8) and transcription (10). These results point to an altered transcriptome in SMCs of the C57Bl/6 aorta at an atherosclerosis-prone location. This is in agreement with findings in endothelial cells in atherosclerosis-prone regions. It could be due to biomechanical differences, for instance in wall tension or shear stress, or the different embryological origin of SMCs in AA and TA.
 
Overall design Samples were hybridized in dye-swap design.
 
Contributor(s) Van Assche T, Hendrickx J, Guns P, Martinet W, Fransen P, Bult H
Citation(s) 20606469
Submission date Dec 05, 2008
Last update date Dec 06, 2012
Contact name Rekin's Janky
E-mail(s) Nucleomics.Bioinformatics@vib.be
Organization name VIB
Department Nucleomics Core
Street address Herestraat 49 Box 816
City Leuven
ZIP/Postal code B-3000
Country Belgium
 
Platforms (1)
GPL2872 Agilent-012694 Whole Mouse Genome G4122A (Feature Number version)
Samples (2)
GSM348025 Ag-Mo-WG-G4122A: hyb 2738
GSM348026 Ag-Mo-WG-G4122A: hyb 2739
This SubSeries is part of SuperSeries:
GSE13865 Smooth muscle cells in atherosclerosis-prone and resistant regions of aorta
Relations
BioProject PRJNA114585

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13835_RAW.tar 27.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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