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Status |
Public on May 15, 2009 |
Title |
Smooth muscle cells in atherosclerosis-prone and resistant regions of the aorta of C57Bl/6 mice at age of 4 months |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
This study compared gene expression in smooth muscle cells (SMCs) in atherosclerosis-prone and atherosclerosis-resistant regions of the aorta of C57Bl/6 mice. In a parallel experiment, both regions were compared in young, plaque-free apolipoprotein E-deficient (apoE-/-) mice. Aortas of 3 male and 3 female C57Bl6 mice were isolated, perfused with triton X-100 to remove endothelial cells and divided in an atherosclerosis-prone region (AA: ascending aorta, aortic arch and proximal 2 mm of thoracic aorta) and an atherosclerosis-resistant region (TA: central thoracic aorta,i.e. 6 mm distal from the proximal 2mm of the thoracic aorta). Microarray analysis (VIB-MAF) of pooled total RNA showed differential expression (>2-fold difference) for 70 genes. Up- or downregulation in the AA was observed for 33 and 37 genes respectively. Differential expression of 3 genes (ATPase, Na+/K+ transporting, beta 1 polypeptide, sarcolipin and homeo box B7) was confirmed using real-time PCR. Twenty five genes showed exclusively differential expression in C57BL6 mice. Only 7 could be linked to specific processes: development (4) and cell growth (3). The other 18 genes were all involved in different processes. Among the 45 genes showing differential expression in C57Bl/6 as well as apoE-/- mice, most were related to development (13), cell growth (8) and transcription (10). These results point to an altered transcriptome in SMCs of the C57Bl/6 aorta at an atherosclerosis-prone location. This is in agreement with findings in endothelial cells in atherosclerosis-prone regions. It could be due to biomechanical differences, for instance in wall tension or shear stress, or the different embryological origin of SMCs in AA and TA.
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Overall design |
Samples were hybridized in dye-swap design.
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Contributor(s) |
Van Assche T, Hendrickx J, Guns P, Martinet W, Fransen P, Bult H |
Citation(s) |
20606469 |
Submission date |
Dec 05, 2008 |
Last update date |
Dec 06, 2012 |
Contact name |
Rekin's Janky |
E-mail(s) |
Nucleomics.Bioinformatics@vib.be
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Organization name |
VIB
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Department |
Nucleomics Core
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Street address |
Herestraat 49 Box 816
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City |
Leuven |
ZIP/Postal code |
B-3000 |
Country |
Belgium |
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Platforms (1) |
GPL2872 |
Agilent-012694 Whole Mouse Genome G4122A (Feature Number version) |
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Samples (2) |
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This SubSeries is part of SuperSeries: |
GSE13865 |
Smooth muscle cells in atherosclerosis-prone and resistant regions of aorta |
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Relations |
BioProject |
PRJNA114585 |
Supplementary file |
Size |
Download |
File type/resource |
GSE13835_RAW.tar |
27.4 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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