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| Status |
Public on Dec 17, 2019 |
| Title |
Changes in calcium homeostasis and gene expression implicated in epilepsy in hippocampi of mice overexpressing ORAI1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We showed that the overexpression of ORAI1 in the neurons of murine brain leads to spontaneous occurrence of seizure-like events in aged animals. We aimed to identify the mechanism responsible for this phenomenon. Using modified Ca2+-addback assay in the slices of CA1 hippocampal region and FURA-2AM calcium indicator we found that overexpression of ORAI1 in neurons leads to altered Ca2+ response. Next, by RNASeq we identified set of genes, whose expression was changed in FVB/NJ-Tg(ORAI1)Ibd mice.
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| Overall design |
We isolated total RNA from hippocampi of wild-type (n=3) and of FVB/NJ-Tg(Orai1)Ibd (n=3) females and performed RNAseq experiments.
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| Contributor(s) |
Wojtas B, Majewski L |
| Citation(s) |
31698854 |
| Submission date |
Oct 03, 2019 |
| Last update date |
Oct 06, 2023 |
| Contact name |
Bartosz Wojtas |
| E-mail(s) |
bartoszwojtas83@gmail.com
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| Phone |
692156006
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| Organization name |
Nencki Institute of Experimental Biology
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| Street address |
Pasteura 3
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| City |
Warsaw |
| ZIP/Postal code |
02-793 |
| Country |
Poland |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA575645 |
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