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Status |
Public on Sep 01, 2020 |
Title |
Comparison of bioprinted kidney organoids in different conformations |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Bulk RNA-seq comparison of kidney organoids bioprinted in 3 different conformations with varying starting cell densities. Density is dictated by the ratio of bioprinter tip movement to the amount of extrusion, where higher ratios spread cells over a larger surface area. We compare organoids printed with no movement ('blob', ratio 0) to those with moderate ('line 3', ratio 20) or high movement ('line 1', ratio 40).
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Overall design |
Each condition consistes of 3 replicate organoids, where each replicate was generated from a distinct well of differentiated iPSCs.
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Web link |
https://github.com/KidneyRegeneration/BioprintedOrganoids
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Contributor(s) |
Lawlor KT, Vanslambrouck JM, Little MH |
Citation(s) |
33230326 |
Submission date |
Oct 10, 2019 |
Last update date |
Mar 30, 2022 |
Contact name |
Kynan Thomas Lawlor |
E-mail(s) |
kynan.lawlor@mcri.edu.au
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Organization name |
Murdoch Children's Research Institute
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Street address |
Murdoch Children's Research Institute, Flemington Road
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City |
Parkville |
State/province |
VIC |
ZIP/Postal code |
3052 |
Country |
Australia |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (9)
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Relations |
BioProject |
PRJNA576921 |
SRA |
SRP225115 |