|
| Status |
Public on Dec 04, 2020 |
| Title |
Profiling Kupffer cells with different origins in NASH |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Kupffer cells (KCs) self-renew by local proliferation in the adult independently from monocytes (Mos). However, how they maintain during chronic metabolic disorders such as non-alcoholic steatohepatitis (NASH) remains ill-defined. We characterized KCs during NASH and observed diversity in the KC pool, with a significant fraction of monocyte-derived KCs (MoKCs). Here, we aim to uncover the transcriptional landscapes of KCs subsets found in a mouse model of methionine and choline deficient (MCD)-diet induced non-alcoholic steatohepatitis (NASH).
|
| |
|
| Overall design |
Wild-type mice were fed a MCD diet for 4 weeks and Kupffer cell subsets were sorted by flow cytometry. EmKCs were CLEC2+ TIMD4+ while MoKCs were CLEC2+ TIMD4-.
|
| |
|
| Contributor(s) |
Dussaud S, Gautier E |
| Citation(s) |
32562600 |
| Submission date |
Oct 11, 2019 |
| Last update date |
Dec 04, 2020 |
| Contact name |
Sebastien Dussaud |
| E-mail(s) |
sebastien.dussaud@upmc.fr
|
| Organization name |
UPMC
|
| Street address |
91 bd de l'hopital
|
| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (19)
|
|
| Relations |
| BioProject |
PRJNA577115 |
| SRA |
SRP225214 |
Less...
More...