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Series GSE139003 Query DataSets for GSE139003
Status Public on Oct 31, 2020
Title RNA-seq timecourse of p53 activation with Nutlin-3a in IMR90 cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the dynamics of the p53 response, we treated IMR90 cells with Nutlin-3a, a small molecule that inhibits the interaction between p53 and its inhibitor Mdm2, for 6, 9, or 12 hours.
 
Overall design RNA-seq in IMR90 cells treated with Nutlin-3a (inhibitor to Mdm2-p53 interaction) for 6, 9, or 12 hours or treated with a DMSO control.
 
Contributor(s) Alexander KA, Berger SL
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NIH grant(s)
Grant ID Grant title Affiliation Name
F32 CA221010 Modulation of wild type and mutant p53 by HMGA1 UNIVERSITY OF PENNSYLVANIA Katherine Ann Alexander
R01 CA078831 Epigenetic regulation by tumor suppressor p53 UNIVERSITY OF PENNSYLVANIA SHELLEY L BERGER
Submission date Oct 17, 2019
Last update date Oct 31, 2020
Contact name Katherine A Alexander
E-mail(s) kaalexa@pennmedicine.upenn.edu
Organization name University of Pennsylvania
Street address Smilow Center for Translational Research
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (8)
GSM4126024 dmso_rep1
GSM4126025 dmso_rep2
GSM4126026 6hNutlin_rep1
Relations
BioProject PRJNA578044
SRA SRP226017

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139003_DMSOvs12h.txt.gz 946.2 Kb (ftp)(http) TXT
GSE139003_DMSOvs6h.txt.gz 940.9 Kb (ftp)(http) TXT
GSE139003_DMSOvs9h.txt.gz 939.8 Kb (ftp)(http) TXT
GSE139003_IMR90_Nutlin3aTimecourse_normCounts.txt.gz 968.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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