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Status |
Public on Apr 30, 2020 |
Title |
Next Generation sequencing revealed the quantitative transcriptome analysis of the preimplantation embryos (3 d.p.c) generated from the cross between L13a+/- mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Comparative study of the genomewide transcriptome analysis with reference to the regression scale of ribosomal protein L13a transcript level i.e in the absence of L13a (-/-), intermediate level of L13a (+/-) and wild type (+/+)
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Overall design |
Preimplanted embryos at the morula stage were collected by flashing the oviduct/uterus of pregnant L13a(+/-) mice at 3 d.p.c Each embryos were washed in PBS and transferred to 10ml of Cells-to-CDNA II Cell lysis buffer (Cat#AM8723 invitrogen) and boiled at 75 degree Centrigrade for 10 minutes, lysates were centrifuged briefly and transferred immediately on ice. RNA purification was performed using PicoPure RNA isolation it (Cat# KIT0214) following users manual. These samples were submitted for library construction and next generation sequencing.
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Contributor(s) |
Kour R, Richardson B, Cameron M, Mazumder B |
Citation(s) |
37902531 |
Submission date |
Oct 22, 2019 |
Last update date |
Nov 07, 2023 |
Contact name |
Mark Cameron |
E-mail(s) |
mjc230@case.edu
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Organization name |
Case Western Reserve University
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Department |
Population and Quantitative Health Sciences
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Lab |
Cameron
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Street address |
10900 Euclid Ave
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City |
Cleveland |
State/province |
OH |
ZIP/Postal code |
44106 |
Country |
USA |
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Platforms (1) |
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Samples (7)
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GSM4134515 |
Preimplantation Morula embryo from L13a-/+ cross-1 |
GSM4134516 |
Preimplantation Morula embryo from L13a-/+ cross-2 |
GSM4134517 |
Preimplantation Morula embryo from L13a-/+ cross-3 |
GSM4134518 |
Preimplantation Morula embryo from L13a-/+ cross-4 |
GSM4134519 |
Preimplantation Morula embryo from L13a-/+ cross-5 |
GSM4134520 |
Preimplantation Morula embryo from L13a-/+ cross-6 |
GSM4134521 |
Preimplantation Morula embryo from L13a-/+ cross-7 |
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Relations |
BioProject |
PRJNA578866 |
SRA |
SRP226591 |