NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE139241 Query DataSets for GSE139241
Status Public on Apr 30, 2020
Title Next Generation sequencing revealed the quantitative transcriptome analysis of the preimplantation embryos (3 d.p.c) generated from the cross between L13a+/- mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Comparative study of the genomewide transcriptome analysis with reference to the regression scale of ribosomal protein L13a transcript level i.e in the absence of L13a (-/-), intermediate level of L13a (+/-) and wild type (+/+)
 
Overall design Preimplanted embryos at the morula stage were collected by flashing the oviduct/uterus of pregnant L13a(+/-) mice at 3 d.p.c Each embryos were washed in PBS and transferred to 10ml of Cells-to-CDNA II Cell lysis buffer (Cat#AM8723 invitrogen) and boiled at 75 degree Centrigrade for 10 minutes, lysates were centrifuged briefly and transferred immediately on ice. RNA purification was performed using PicoPure RNA isolation it (Cat# KIT0214) following users manual. These samples were submitted for library construction and next generation sequencing.
 
Contributor(s) Kour R, Richardson B, Cameron M, Mazumder B
Citation(s) 37902531
Submission date Oct 22, 2019
Last update date Nov 07, 2023
Contact name Mark Cameron
E-mail(s) mjc230@case.edu
Organization name Case Western Reserve University
Department Population and Quantitative Health Sciences
Lab Cameron
Street address 10900 Euclid Ave
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
 
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (7)
GSM4134515 Preimplantation Morula embryo from L13a-/+ cross-1
GSM4134516 Preimplantation Morula embryo from L13a-/+ cross-2
GSM4134517 Preimplantation Morula embryo from L13a-/+ cross-3
Relations
BioProject PRJNA578866
SRA SRP226591

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139241_normalizedCounts.csv.gz 550.4 Kb (ftp)(http) CSV
GSE139241_rawCounts.csv.gz 234.7 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap