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Series GSE13929 Query DataSets for GSE13929
Status Public on Nov 19, 2009
Title Arabidopsis thaliana three hours after infection with Agrobacterium tumefaciens
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary This study focuses on responses of the host plant to infection with Agrobacterium tumefaciens. Genome wide changes in gene expression were integrated with the alterations in metabolite levels three hours after inoculation of agrobacteria. Plants were infected with the virulent strain C58, harboring a T-DNA, or with strain GV3101, containing a disarmed Ti-plasmid. This allows discrimination between signals which derive from the bacterial pathogen and the T-DNA encoded genes.
 
Overall design The bases of Arabidopsis thaliana (WS-2) inflorescence stalks were wounded and immediately infected with Agrobacterium tumefaciens or mock-infected for three hours. Stalks of intact plants were inoculated with the oncogenic strain C58 (C58 3 hpi 1 to 6) or the non-virulent strain GV3101 (GV3101 3 hpi 1 to 3) to provide conditions close to nature. The gene expression data of three independent experiments of infected material were compared with six non-infected samples (reference 3 hpi 1 to 6). Differential gene expression was determined by applying the LIMMA package (Linear Models for Microarray Data; Smyth, G.K. (2004) Applic. Genet. Mol. Biol. 3, Article 3; http://www.bepress.com/sagmb/vol3/iss1/art3/).
 
Contributor(s) Deeken R, Engelmann J
Citation(s) 17172353, 19794116
Submission date Dec 12, 2008
Last update date Aug 15, 2018
Contact name Rosalia Deeken
E-mail(s) deeken@botanik.uni-wuerzburg.de
Phone +49 931 8886121
Fax +49 931 8886158
Organization name University of Wuerzburg, Julius-von-Sachs-Institute
Department Botany I; Plant Molecular Physiology & Biophysics
Lab Prof. Hedrich
Street address Julius-von-Sachs-Platz 2
City Wuerzburg
ZIP/Postal code 97072
Country Germany
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (15)
GSM350855 Reference 3hpi 1
GSM350856 Reference 3hpi 2
GSM350857 Reference 3hpi 3
This SubSeries is part of SuperSeries:
GSE14106 Transcriptome data from inflorescence stalks of intact A. thaliana plants after infection with A. tumefaciens
Relations
BioProject PRJNA114607

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13929_RAW.tar 30.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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