 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 06, 2020 |
| Title |
Genome-wide CRISPR screen of rituximab induced cell depletion |
| Organisms |
Homo sapiens; unidentified plasmid |
| Experiment type |
Other
|
| Summary |
We screened the genome of OCI-Ly-7, a Diffuse Large B-cell Lymphoma cell line, for genes mediating the response to rituximab treatment. We utilized the genome-wide library GeCKO v2 (A+B) from Feng Zhang. (Addgene#1000000049)
|
| |
|
| Overall design |
In addition to mock treated sample, we subjected cells to two different rituximab conditions. RTX-SEC (short exposure to CDC conditions, SEC, followed by non-CDC conditions for a total of 14 days) and RTX-REC (repeated exposure to CDC conditions, REC, maintained for 21 days). The rationale was to expose cells in the RTX-SEC group shortly to CDC conditions followed by non-CDC conditions, whereas cells in the RTX-REC group were repeatedly subjected to conditions supporting CDC
|
| |
|
| Contributor(s) |
Thomsen EA, Mikkelsen JG |
| Citation(s) |
32585766 |
| Submission date |
Oct 25, 2019 |
| Last update date |
Jul 15, 2020 |
| Contact name |
Jacob Giehm Mikkelsen |
| E-mail(s) |
giehm@biomed.au.dk
|
| Organization name |
Aarhus University
|
| Department |
Biomedicin
|
| Lab |
Jacob Giehm Mikkelsen
|
| Street address |
Bartholins Allé 6
|
| City |
Aarhus |
| ZIP/Postal code |
8000 Aarhus C |
| Country |
Denmark |
| |
|
| Platforms (2) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA579532 |
| SRA |
SRP226990 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE139385_RTX_screen_mageck.count.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...