|
| Status |
Public on Aug 25, 2020 |
| Title |
Analysis of Circulating Breast Cancer Cell Heterogeneity and Interactions with Peripheral Blood Mononuclear Cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To infer interactions between circulating breast cancer cells and peripheral mononuclear cells using bioinformatics analysis of single cell RNA-sequencing.
Methods: Filter based method for capturing single cells from 7.5mL of blood from patients with breast cancer, followed by single cell RNA-sequencing.
Results: Transcriptomes predicted for two CTC populations with distinct expression profiles and interactions with peripheral blood mononuclear cells.
|
| |
|
| Overall design |
10 samples from breast cancer patients (9 with metastatic breast cancer and 1 with stage 2 local breast cancer). A total of 7.5 mL of blood from each sample was used to capture single cells for single cell RNA-sequencing.
|
| |
|
| Contributor(s) |
Kabos P, Brechbuhl HM, Paul KV, Gillen AE |
| Citation(s) |
32822091 |
| Submission date |
Oct 28, 2019 |
| Last update date |
Feb 27, 2024 |
| Contact name |
Kiran Vinod Paul |
| E-mail(s) |
kiran.vinodpaul@cuanschutz.edu
|
| Organization name |
University of Colorado-Anschutz medical campus
|
| Street address |
12631 E 17th Ave
|
| City |
Aurora |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA579983 |
| SRA |
SRP227241 |
Less...
More...