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Series GSE139942 Query DataSets for GSE139942
Status Public on Jun 24, 2020
Title ChIP-seq profiling of myogenin and p300 occupancy in the context of rexinoid signaling during early myogenic differentiation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Deciphering the molecular mechanisms underpinning myoblast differentiation is a critical step in developing the best strategy to promote muscle regeneration in patients suffering from muscle-related diseases. We have previously established that a rexinoid x receptor (RXR)-selective agonist enhances the differentiation and fusion of myoblasts through a direct regulation of MyoD expression, coupled with an augmentation of myogenin protein. Here, we found that RXR signaling modifies the chromatin state distribution of myogenin, promoting the binding preference of myogenin for poised enhancers and a distinct motif. We also found an association of myogenin with rexinoid-responsive gene expression and identified an epigenetic signature related to histone acetyltransferase p300. Moreover, RXR signaling instigates residue-specific histone acetylation at enhancers co-occupied by p300 and myogenin. Thus, genomic distribution of transcriptional regulators is an important designate for identifying novel targets as well as developing therapeutics that modulate epigenetic landscape in a selective manner to promote muscle regeneration.
Overall design ChIP-seq of myogenin, histone acetyltransferase p300, and H3K27me3 in C2C12 myoblasts differentiated for 24 hours in the absence or presence of bexarotene. Untreated differentiating myoblasts and input chromatin DNA were used as controls.
Contributor(s) Khilji S, Hamed M, Li Q
Citation(s) 32555436, 34603059
Submission date Nov 05, 2019
Last update date Oct 06, 2021
Contact name Qiao Li
Organization name University of Ottawa
Department Cellular and Molecular Medicine
Street address 451 smyth road
City Ottawa
State/province Ontario
ZIP/Postal code K1H 8M5
Country Canada
Platforms (3)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM4150361 C2C12_Input_p300_Histones
GSM4150362 C2C12_p300_Ctl
GSM4150363 C2C12_p300_Bex
BioProject PRJNA587703
SRA SRP228589

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Supplementary file Size Download File type/resource
GSE139942_RAW.tar 544.8 Mb (http)(custom) TAR (of BEDGRAPH, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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