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| Status |
Public on Nov 06, 2019 |
| Title |
ATAC-seq profile of mouse palatal epithelium at E14.5 |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To profile nucleosome free regions of moust palatal epithelium at E14.5, we performed ATAC-seq for isolated palatal epithelium and mesenchyme cells.
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| Overall design |
We digested the mouse embryonic palate at E14.5 with 0.25% trypsin-EDTA (Life Technologies) at 4°C for 10 minutes, after which the reaction was stopped using trypsin Inhibitor (Life Technologies). Under a dissecting microscope, the epithelium was isolated for ATAC-seq by gently peeling using microforceps. The remaining tissue, comprised largely of mesenchymal cells, was also collected for control samples. For each biological replicates, 20000 cells from 3 different mouse embryos were used. Three biological replicates were sequenced using HiSeq X Ten.
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| Contributor(s) |
Liu H, Cornell RA |
| Citation(s) |
32031521 |
| Submission date |
Nov 05, 2019 |
| Last update date |
Oct 07, 2020 |
| Contact name |
Robert Cornell |
| E-mail(s) |
robert-cornell@uiowa.edu
|
| Organization name |
University of Iowa
|
| Department |
Anatomy and Cell Biology
|
| Street address |
1-400 D Bowen Science Bldg. 51 Newton Road
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| City |
Iowa City |
| State/province |
Iowa |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA587709 |
| SRA |
SRP228592 |
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