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| Status |
Public on Oct 25, 2020 |
| Title |
Molecular atlas of fetal and adult human liver sinusoidal endothelial cells: a F8 secreting cell [methylation I] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
In human F8 deficiency leads to hemophilia A and is largely synthesized and secreted by the sinusoidal endothelial cells of the liver (LSECs). However, the specificity and characteristics of these cells is not well known. In this study, we performed genome wide expression and CpG methylation profiling of fetal and adult human LSECs together with other fetal endothelial cells, from lung (micro-vascular and arterial), and heart (micro-vascular). Our results reveal plenty of expression and methylation markers distinguishing LSECs at both fetal and adult stages. Differential gene expression of fetal LSECs in comparison to other fetal endothelial cells pointed to several differential regulated pathways and functions in fetal LSECs. We used targeted bisulfite re-sequencing to confirm selected top differentially methylated regions. We further designed an assay where we used the selected methylation markers to test the degree of similarity of in house iPSs generated vascular endothelial cells to fetal and adult LSECs; a higher similarity was found to the fetal than to adult LSECs. Our molecular profiling study provides a guide to test the effectiveness of production of in vitro differentiated LSECs that could be used in cellular therapies.
In-silico analysis to identify molecular signature of liver sinusoidal endothelial cells in comparison to other endohtelial cells
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| Overall design |
Commercial endothelial cells were ordered from ScienCell research laboratories via Provitro GmbH. All molecular materials were isolated at passage one as stated by manufacturing certificate.
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| Web link |
https://0-www-mdpi-com.brum.beds.ac.uk/1422-0067/21/20/7776/s1
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| Contributor(s) |
Jamil M, El-Maarri O |
| Citation(s) |
33096636 |
| Submission date |
Nov 07, 2019 |
| Last update date |
Oct 27, 2020 |
| Contact name |
Muhammad Ahmer Jamil |
| Organization name |
University Clinic Bonn
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| Department |
Institute of experimental haematology and transfusion medicine
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| Street address |
Sigmund-Freud Str. 25
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| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53127 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE140079 |
Molecular Analysis of Fetal and Adult Primary Human Liver Sinusoidal Endothelial Cells: A Comparison to Other Endothelial Cells |
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| Relations |
| BioProject |
PRJNA588188 |
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