|
| Status |
Public on Feb 01, 2021 |
| Title |
Novel Isoforms of the Epigenetic Regulator TET1 Differentially Control CNS Synaptic Function, Plasticity, and Cognition |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
Two distinct Tet1 isoforms are expressed, one encoding full-length TET1 (Tet1L) and the other a truncated transcript (Tet1S), lacking a large portion of the N-terminus. Each isoform displays unique transcriptional regulation, subcellular protein localization and enzymatic activity. In neurons, Tet1L and Tet1S govern the expression and methylation levels of distinct gene ensembles that when disrupted.
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| Overall design |
In mouse hippocampal cultured neurons, Tet1L and Tet1S were selectively overexpressed and downregulated to identify loci of differential DNA methylation and gene expression.
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| Contributor(s) |
Kennedy AJ, Greer CB, Kaas GA |
| Citation(s) |
33262245 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P20 GM103423 |
Comparative Functional Genomics INBRE in Maine |
MDI Biological Laboratory |
JAMES A COFFMAN |
| R01 MH057014 |
Biochemical Mechanisms of Neural Plasticity |
Vanderbilt University Medical Center |
DANNY G. WINDER |
|
| Submission date |
Nov 08, 2019 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Andrew John Kennedy |
| E-mail(s) |
akennedy@bates.edu
|
| Organization name |
Bates College
|
| Department |
Chemistry
|
| Lab |
Kennedy
|
| Street address |
5 Andrews Rd, Dana 322
|
| City |
Lewiston |
| State/province |
ME |
| ZIP/Postal code |
04240 |
| Country |
USA |
| |
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| Platforms (2) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA588473 |
| SRA |
SRP229373 |
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