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Series GSE14091 Query DataSets for GSE14091
Status Public on Dec 23, 2008
Title Identification of ANAC059 and ANAC092 early target genes
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Identification of ANAC059 and ANAC092 early target genes using inducible overexpression system coupled with investigation of T-DNA insertion mutants
The aim was to identify early target genes of two senescence-associated transcription factors: ANAC059 and ANAC092. For this purpose we used estradiole-inducible system and studied the expression profile 5h after treatment using Affymetrix microarray. Additionally expression profile of T-DNA insertion lines for ANAC059, ANAC092 and the wild-type were also analyzed.
Arabidopsis three weeks old transgenic plants (inducible overexpression) were treated with estradiole for 5h. Based on our preliminary expriments we could identify that 5-h treatment is the shortest time point which result to the induction of ANAC092 and ANAC059 in inducible ANAC092 and ANAC059 overexpression plants, respectively. Additionally leaves of 38 days old plants were harvest in T-DNA insertion lines of ANAC059, ANAC92 and the wild-type and expression profile was performed.

Keywords: genetic modification
 
Overall design 3weeks old Arabidopsis ANAC059 and ANAC092 estradiole-induced overexpresion plants were treated with estradiole for 5h. As control, empty vector transgenic plants were also treated as same condition. Shoots of treated plants were harvested in order to RNA extraction and hybridization to affymetrix GeneChip array. For T-DNA insertion mutants, leaf number 11 were harvested 38 days old wt and mutant plants.
 
Contributor(s) Balazadeh S, Mueller-Roeber B
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Submission date Dec 22, 2008
Last update date Aug 28, 2018
Contact name Salma Balazadeh
E-mail(s) balazadeh@mpimp-golm.mpg.de
Organization name MPIMP
Department Stitt
Lab Mueller-Roeber
Street address Am Muehlenberg 1
City Postdam
ZIP/Postal code 14476
Country Germany
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (13)
GSM353584 3 weeks old transgenic plants (empty vector; inducible system), biological rep1
GSM353585 3 weeks old transgenic plants (empty vector; inducible system), biological rep2
GSM353586 3 weeks old transgenic plants (empty vector; inducible system), biological rep3
Relations
Affiliated with GSE69995
BioProject PRJNA112521

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Supplementary file Size Download File type/resource
GSE14091_RAW.tar 119.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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