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Series GSE14096 Query DataSets for GSE14096
Status Public on Dec 25, 2008
Title Urinary bladder post-spinal cord injury: time-points
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Previously, we demonstrated using a rat model of spinal cord injury (SCI) that bladder wall tissue compliance significantly increased within the first 2 weeks following injury. In order to explore the potential molecular-level mechanisms of this event, the present study quantified molecules pertinent to bladder tissue remodeling and changes in mechanical properties. An initial gene array analysis followed by real-time qPCR revealed that the message levels for tropoelastin and lysyl oxidase were as high as 8-fold in SCI rats compared to normal. Furthermore, both the message and protein levels of TGF-beta1 and IGF-1, known stimulators of elastin synthesis, in SCI rat bladders were significantly higher compared to those of normal rats. Taken together, it can be speculated that functional changes of the bladder associated with SCI induce release of select growth factors, which, in turn, stimulate elastogenesis that lead to alteration of biomechanical properties of the wall tissue.
 
Overall design One bladder in each of the four groups. One normal control, one 3 days post-SCI, one 7 days, and one 25 days. Only one sample per group for initial screening analysis.
The microarray data was used in an additional study using Ingenuity Pathways Analysis to elucidate remodeling, inflammation and infection events.
 
Contributor(s) Nagatomi J, DeMiguel F, Torimoto K, Chancellor MB, Getzenberg RH, Sacks MS, Wognum S, Lagoa CE, Vodovotz Y
Citation(s) 16038877
Submission date Dec 22, 2008
Last update date Feb 21, 2017
Contact name Silvia Wognum
Organization name University of Pittsburgh
Department Bioengineering
Lab ETM2L
Street address 100 Technology Drive
City Pittsburgh
State/province PA
ZIP/Postal code 15219
Country USA
 
Platforms (1)
GPL85 [RG_U34A] Affymetrix Rat Genome U34 Array
Samples (4)
GSM353862 ratbladder_normalcontrol
GSM353864 ratbladder_SCI_3days
GSM353865 ratbladder_SCI_7days
Relations
BioProject PRJNA112385

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14096_RAW.tar 18.3 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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