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Series GSE140979 Query DataSets for GSE140979
Status Public on Oct 26, 2021
Title K27M and K36M mutations on non canonical Histone 3.3 promote redistribution of antagonistic chromatin marks, disrupted development, and tumorigenesis
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We conducted RNA-seq analysis to identify genes that are differentially expressed in H3K27M and H3K36M oncohistones compared to wt and control eye tissue (eye discs), followed by smRNA-seq profiling based on our results and to confirm upregulation of piRNAs
 
Overall design 3 data types (RNA-seq, smRNA-seq and ChIP-seq data). RNA-seq and smRNA-seq experiments has 12 samples, which is composed of 3 replicates of 4 genotypes (H3K27M, H3K36M, histone WT and control eye tissue (Yw)). Additional RNA-seq RNAi experiments has been performed on 10 sets (3 replicates each, 30 samples total). H3K27me3 and H3K36me2 ChIP-seq was also performed (14 samples)
 
Contributor(s) Chaouch A, Berlandi J, Frey F, Chen CC, Badini S, Marchione D, Garcia B, Harutyunyan A, Krug B, Chen H, Majewski J, Hébert S, Jeibmann A, Kleinman CL, Hasselblatt M, Lasko P, Shirinian M, Jabado N
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Submission date Nov 25, 2019
Last update date Oct 30, 2021
Contact name Claudia L Kleinman
E-mail(s) claudia.kleinman@mcgill.ca
Phone 514-340-8222 26129
Organization name Lady Davis Institute for Medical Research
Department Human Genetics
Street address 3999 Côte Ste-Catherine Road
City Montréal
State/province Québec
ZIP/Postal code H3T 1E2
Country Canada
 
Platforms (2)
GPL21306 Illumina HiSeq 4000 (Drosophila melanogaster)
GPL25244 Illumina NovaSeq 6000 (Drosophila melanogaster)
Samples (68)
GSM4191832 smRNA-K27MxEyG4_prep1
GSM4191833 smRNA-K27MxEyG4_prep2
GSM4191834 smRNA-K27MxEyG4_prep3
Relations
BioProject PRJNA591601
SRA SRP233209

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140979_RAW.tar 5.3 Gb (http)(custom) TAR (of BEDGRAPH, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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