|
| Status |
Public on Jun 02, 2021 |
| Title |
Inflammation drives alternative first exon usage of critical immune genes including Aim2 [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Macrophage cells are essential components of the innate immune system and contain toll-like receptors (TLRs) that intiate the immune response when pathogen-associated molecular pattern molecules (PAMPs) are recognized.
In this experiment, we stimulate TLR4 in bone marrow-derived macrophage cells with lipopolysaccharide (LPS) for 6 hours to simmulate inflammation and study the resulting differential splicing landscape and gene expression.
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| |
|
| Overall design |
Unstimulated or LPS stimulated Primary Bone Marrow Derived Macrophages (BMDMs) from WT mice were collected and placed in Trizol. RNA was isolated and sequenced using Illumina.
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| |
|
| Contributor(s) |
Carpenter S, Brooks A |
| Citation(s) |
34047695 |
| Submission date |
Dec 09, 2019 |
| Last update date |
Sep 01, 2021 |
| Contact name |
Sergio Covarrubias |
| E-mail(s) |
secovarr@ucsc.edu
|
| Phone |
2132710156
|
| Organization name |
UCSC
|
| Department |
MCD
|
| Lab |
Carpenter
|
| Street address |
Thimann Receiving/Department Carpenter Lab/Biomed 125, 1156 High St., Thimann Labs
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE141754 |
Inflammation drives alternative first exon usage of critical immune genes including Aim2 |
|
| Relations |
| BioProject |
PRJNA594369 |
| SRA |
SRP235279 |
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