 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 11, 2019 |
| Title |
Gene expression changes in IDH mutant or IDH wildtype gliomas treated with All-trans Retinoic Acid |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
IDH mutant cells are deficient in retinoic acid production, which impacts downstream gene expression and pathways. We looked at the effect of supplementing IDH mutant glioma cells with ATRA
on gene expression and downstream pathways. We compared gene expression changes with IDH WT cells that were also treated with ATRA
|
| |
|
| Overall design |
Primary IDH mutant/ wildtype glioma cells were cultured in vitro and treated with 1uM ATRA for 48 hours. DMSO treated cells were used as controls. After treatment, RNA was extracted from cells and sent for microarray analysis at the Genomics core at the University of Pittsburgh
|
| |
|
| Contributor(s) |
Amankulor N, Rao A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 10, 2019 |
| Last update date |
Dec 13, 2019 |
| Contact name |
Nduka Amankulor |
| E-mail(s) |
APR15@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Street address |
5117 Center Avenue
|
| City |
Pittsburgh |
| ZIP/Postal code |
15232 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
|
| Samples (12)
|
| GSM4213281 |
IDH WT glioma cells treated with DMSO, Replicate 1 |
| GSM4213282 |
IDH WT glioma cells treated with DMSO, Replicate 2 |
| GSM4213283 |
IDH WT glioma cells treated with DMSO, Replicate 3 |
| GSM4213284 |
IDH WT glioma cells treated with ATRA, Replicate 1 |
| GSM4213285 |
IDH WT glioma cells treated with ATRA, Replicate 2 |
| GSM4213286 |
IDH WT glioma cells treated with ATRA, Replicate 3 |
| GSM4213287 |
IDH MUT glioma cells treated with DMSO, Replicate 1 |
| GSM4213288 |
IDH MUT glioma cells treated with DMSO, Replicate 2 |
| GSM4213289 |
IDH MUT glioma cells treated with DMSO, Replicate 3 |
| GSM4213290 |
IDH MUT glioma cells treated with ATRA, Replicate 1 |
| GSM4213291 |
IDH MUT glioma cells treated with ATRA, Replicate 2 |
| GSM4213292 |
IDH MUT glioma cells treated with ATRA, Replicate 3 |
|
| Relations |
| BioProject |
PRJNA594739 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE141789_RAW.tar |
14.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...