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Status |
Public on Sep 29, 2020 |
Title |
Identification of potential target genes of KRAB zinc-finger proteins in cancer cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
KRAB zinc-finger proteins (KZFPs) are potent transcriptional suppressors and suspected to be associated with cancer progression. To identify the potential target genes of KZFPs in lung adenocarcinoma cells, we established a panel of A459 cell lines that over-express 30 types of KZFPs (A549/KZFP cells) and performed RNA-sequencing (RNA-Seq) analysis of these cells.
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Overall design |
The lentivirus vector expressing HA-tagged KZFP was transduced into A459 cells. The ectopic expression of KZFP in A549/KZFP cells was verified by Western blotting with an anti-HA antibody. Total RNA was extracted from the A549/KZFP cells by QIAamp RNA Blood Mini Kit (QIAGEN #52304) and treated with RNase-Free DNase Set (QIAGEN #79254). Quality check of the purified RNA, library construction, and sequencing were performed by Novogene (https://en.novogene.com). Pair-ended and 150bp read length sequencing was performed by Illumina Novaseq6000. Single-replicate experiment was performed for respective A549/KZFP cells, and triplicate experiment was performed for the empty vector-transduced A549 cells.
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Contributor(s) |
Kimura I, Misawa N, Ito J |
Citation(s) |
33087347 |
Submission date |
Dec 10, 2019 |
Last update date |
Nov 09, 2020 |
Contact name |
Kei Sato |
E-mail(s) |
su9ark@gmail.com
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Phone |
81757514813
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Organization name |
Kyoto University
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Department |
Institute for Frontier Life and Medical Sciences
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Lab |
Laboratory of Systems Virology
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Street address |
53 Shogoinkawara-cho, Sakyo-ku
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City |
Kyoto |
State/province |
Kyoto |
ZIP/Postal code |
6068507 |
Country |
Japan |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (33)
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This SubSeries is part of SuperSeries: |
GSE141803 |
Roles of human endogenous retroviruses and KRAB-zinc finger proteins in tumor progression |
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Relations |
BioProject |
PRJNA594761 |
SRA |
SRP235680 |