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| Status |
Public on Aug 31, 2020 |
| Title |
murine jejunal and ileal epithelial tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptional profiling of mouse jejunal epithelia comparing Tcrbd-/-, DAOwt/wt mouse and Tcrbd-/-, DAOG181R/G181R
Intestinal microbiota produce D-amino acids, which are bacteria-specific metabolites, for regulation of bacterial cell wall integrity. Host intestine releases D-amino acid oxidase (DAO) to degrade bacterial D-amino acids, which shapes gut microbial community. However, it is not clarified whether bacterial D-amino acids affect host s immunity. In the present study, we compared mRNA expression in ileal epithelial tissue of DAO null mice with that of control mice in the absence of both T cell receptor beta and delta (Tcrb/d). Transcriptome analysis revealed up-regulation of inflammatory cytokines such as TNFa, IL1b, and IFNg in the epithelial tissue. Up-regulation of such cytokines could promote survival of na ve B cells and increase IgA-producing plasma cells, which in turn results in enhancement of IgA production. These results indicate that DAO controls intestinal immune responses through regulation of na ve B cells and differentiation of mature B cells.
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| Overall design |
Total RNA from 5 mice of each genotype were mixed.
Epithelial tissue of jejunum and ileum from Two genotype, Tcrbd-/-, DAOwt/wt vs. Tcrbd-/-, DAOG181R/G181R, Total RNA from 5 mice were mixed in one sample.
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| Contributor(s) |
Suzuki M, Sasabe J |
| Citation(s) |
33658193 |
| Submission date |
Dec 16, 2019 |
| Last update date |
Mar 16, 2021 |
| Contact name |
Masataka Suzuki |
| E-mail(s) |
masataka.s@keio.jp
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| Phone |
+81-3-5363-3750
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| Organization name |
KEIO University, School of Medicine
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| Department |
Pharmacology
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| Lab |
6S1, Research Park
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| Street address |
35, Shinanomachi, Shinjuku-ku
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| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA595855 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142070_RAW.tar |
60.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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