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| Status |
Public on Oct 27, 2021 |
| Title |
Gene expression profiling of murine renal adenocarcinoma cells during recursive implantations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The process of serial implantation of a cell line in vivo is a known method to render a cell line increasingly aggressive. In this study, we leveraged this technique to generate increasingly aggressive sub-lines of RENCA, a mouse renal carcinoma cell line. We used different implantation strategies to dissect the different stages of tumor progression and metastasis. Cell-line derived RNA and DNA were used to analyze transcriptional, DNA and methylome profiles of the cell types, and to generate signatures able to predict global, disease-free and progression-free survival time in human patients.
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| Overall design |
The mouse renal carcinoma cell line RENCA? were implanted orthotopically under the kidney capsule in female BALB/c mice. We adapted this model to produce new sub-cell lines with progressively enhanced aggressiveness and metastatic potential with differing characteristics. We serially passaged the cell lines using different implantation strategies designed to replicate different aspects of tumor development and metastasis. After each passage, primary tumor cells were purified from organ explant cultures to generate new sub-cell lines. The three injections modes were as follows:
(1) Orthotopic injection under the renal capsule leading to formation of a primary tumor (Kidney group). The primary tumor is explanted and cell lines purified for re-implantation into the kidney.
(2) Intravenous injection (tail vein group). Tumor cells are injected directly into the blood stream leading to formation of tumors in the lungs in the absence of a tumor in the kidney
(3) Orthotopic injection under the renal capsule followed by metastasis to the lungs (Lung group). Tumor cells are purified from the lung metastases and are re-implanted under the kidney capsule for the subsequent passage.
The sub-cell lines derived as described above were sequentially passaged 6 times in vivo using multiple mice per injection mode and per passage to give a total of 67 newly-derived sub-cell lines.
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| Contributor(s) |
Clarke K, Bikfalvi A |
| Citation(s) |
34670568, 36007057 |
| Submission date |
Dec 16, 2019 |
| Last update date |
Sep 15, 2022 |
| Contact name |
Kim Clarke |
| Organization name |
University of Liverpool
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| Street address |
Crown Street
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| City |
Liverpool |
| ZIP/Postal code |
L69 7ZB |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (70)
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| Relations |
| BioProject |
PRJNA595922 |
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