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| Status |
Public on Feb 24, 2021 |
| Title |
Gene expression profiling of immune cells from patients with acute on chronic liver failure (ACLF) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Whole blood from 7 patients with stable cirrhosis, 7 patients with acutely decompensated cirrhosis without ACLF and 17 patients with ACLF (8 related to sepsis and 9 unrelated to sepsis) was sampled into tempus tubes (Applied biosystems, Ambion). The same method was used to sample whole blood from 7 healthy subjects and from 8 patients with septic shock without cirrhosis that were used as comparators.RNA was then extracted using the PerfectPure RNA blood kit (3 PRIME) according to manufactuter instructions.
100 ng of RNA per sample were then hybridized on Human Transcriptome Array 2.0
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| |
|
| Overall design |
Prospective trial comparing whole blood transcriptome of different group of patients according to the existance of chronic liver disease and to its severity
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| |
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| Contributor(s) |
Moreau R, Weiss E, de la Grange P |
| Citation(s) |
33613548 |
| Submission date |
Dec 18, 2019 |
| Last update date |
Feb 24, 2021 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
pierre.delagrange@genosplice.com
|
| Organization name |
GenoSplice technology
|
| Department |
Paris Biotech Santé
|
| Street address |
29, rue du Faubourg Saint-Jacques
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (46)
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| This SubSeries is part of SuperSeries: |
| GSE142256 |
Gene expression profiling patients with cirrhosis and acute on chronic liver failure (ACLF) |
|
| Relations |
| BioProject |
PRJNA596432 |
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