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| Status |
Public on Dec 21, 2019 |
| Title |
Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC, also known as ASC) |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The effect of standard tissue culture on AdMSC is not well defined, therefore we sought to analyse global changes in microRNA expression when a defined ex vivo population of AdMSC was subjected to standard tissue culture conditions.
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| |
|
| Overall design |
A cocktail of antibodies and immunomagnetic beads were used to enrich for untouched ex vivo AdMSC from the stromal vascular fraction of adipose tissue. We used microarrays to detail the global changes in microRNA expression when these cells are freshly sorted and compared to when these cells are grown in standard tissue culture conditions for different time periods
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| |
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| Contributor(s) |
Sheppard HM |
| Citation(s) |
32098272 |
| Submission date |
Dec 20, 2019 |
| Last update date |
Mar 22, 2020 |
| Contact name |
Hilary Sheppard |
| E-mail(s) |
h.sheppard@auckland.ac.nz
|
| Organization name |
Auckland University
|
| Street address |
Symonds St
|
| City |
Auckland |
| ZIP/Postal code |
1050 |
| Country |
New Zealand |
| |
|
| Platforms (1) |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
|
| Samples (12)
|
| GSM4227278 |
Freshly sorted cells, biological rep 1 |
| GSM4227279 |
Sorted cells cultured for 3 days, biological rep 1 |
| GSM4227280 |
Sorted cells cultured for 28 days, biological rep 1 |
| GSM4227281 |
Sorted cells cultured for 9 passages, biological rep 1 |
| GSM4227282 |
Freshly sorted cells, biological rep 2 |
| GSM4227283 |
Sorted cells cultured for 3 days, biological rep 2 |
| GSM4227284 |
Sorted cells cultured for 28 days, biological rep 2 |
| GSM4227285 |
Sorted cells cultured for 9 passages, biological rep 2 |
| GSM4227286 |
Freshly sorted cells, biological rep 3 |
| GSM4227287 |
Sorted cells cultured for 3 days, biological rep 3 |
| GSM4227288 |
Sorted cells cultured for 28 days, biological rep 3 |
| GSM4227289 |
Sorted cells cultured for 9 passages, biological rep 3 |
|
| Relations |
| BioProject |
PRJNA597007 |
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