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| Status |
Public on Jul 28, 2020 |
| Title |
PRMT5 promotes cancer cell migration and invasion through the E2F pathway |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We identified distinct sets of genes under the control of PRMT5 and E2F1. Some of the most highly regulated genes, such as cortactin/CTTN, influenced cell migration, invasion and adherence. We characterised the functional role of the cortactin/CTTN gene, which enabled PRMT5 through E2F1 to promote cellular migration and invasion, whilst decreasing cellular adherence. Most significantly, there was a striking coincidence between the expression of PRMT5 and E2F1 in certain human tumours, and elevated levels of PRMT5, E2F1 and cortactin/CTTN correlated with poor prognosis disease. Our results suggest a causal relationship between PRMT5, E2F1 and the migration and invasion of cancer cells, thereby highlighting an important pathway that contributes to the cancer biology of tumour cells.
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| Overall design |
Examination of expresion profile of colorectal cancer wild type and E2F1 knockdown HCT116 cells treated with PRMT5 inhibitor
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| Contributor(s) |
Barczak W, Jin L, Carr SM, Munro S, Kanapin A, Samsonova A, La Thangue N |
| Citation(s) |
32709847 |
| Submission date |
Dec 20, 2019 |
| Last update date |
Jul 28, 2020 |
| Contact name |
Alexander Kanapin |
| E-mail(s) |
a.kanapin@gmail.com
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| Phone |
+78123636939
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| Organization name |
St Petersburg Universoty
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| Department |
Institute for Translational Biomedicine
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| Street address |
7/9 University Emb.
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| City |
St. Petersburg |
| ZIP/Postal code |
199034 |
| Country |
Russia |
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| Platforms (1) |
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| Samples (12)
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| GSM4227293 |
WT T1-44 cell line RNA-seq replicate 1 |
| GSM4227294 |
WT T1-44 cell line RNA-seq replicate 2 |
| GSM4227295 |
WT T1-44 cell line RNA-seq replicate 3 |
| GSM4227296 |
E2F1Cr cell line RNA-seq replicate 1 |
| GSM4227297 |
E2F1Cr cell line RNA-seq replicate 2 |
| GSM4227298 |
E2F1Cr cell line RNA-seq replicate 3 |
| GSM4227299 |
E2F1Cr T1-44 cell line RNA-seq replicate 1 |
| GSM4227300 |
E2F1Cr T1-44 cell line RNA-seq replicate 2 |
| GSM4227301 |
E2F1Cr T1-44 cell line RNA-seq replicate 3 |
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| Relations |
| BioProject |
PRJNA597013 |
| SRA |
SRP238361 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142430_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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