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| Status |
Public on Aug 25, 2021 |
| Title |
Cerebral cortex and blood transcriptome changes in mouse neonates prenatally exposed to air pollution particulate matter |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: Prenatal exposure to air pollutants is associated with increased risk for neurodevelopmental and neurodegenerative disorders. However, few studies have identified transcriptional changes related to air pollutant exposure.
Methods: RNA sequencing was used to examine transcriptomic changes in blood and cerebral cortex of three male and three female mouse neonates prenatally exposed to traffic-related nano-sized particulate matter (nPM) compared to three male and three female mouse neonates prenatally exposed to control filter air.
Results: We identified 19 nPM-associated differentially expressed genes (nPM-DEGs) in blood and 124 nPM-DEGs in cerebral cortex. The cerebral cortex transcriptional responses to nPM suggested neuroinflammation involvement, including CREB1, BDNF, and IFN γ genes. Both blood and brain tissues showed nPM transcriptional changes related to DNA damage, oxidative stress, and immune responses. Three blood nPM-DEGs showed a canonical correlation of 0.98 with 14 nPM-DEGS in the cerebral cortex, suggesting a convergence of gene expression changes in blood and cerebral cortex. Exploratory sex-stratified analyses suggested a higher number of nPM-DEGs in female cerebral cortex than male cerebral cortex. The sex-stratified analyses identified 2 nPM-DEGs (Rgl2 and Gm37534) shared between blood and cerebral cortex in a sex-dependent manner.
Conclusions: Our findings suggest that prenatal nPM exposure induces transcriptional changes in the cerebral cortex, some of which are also observed in blood. Further research is needed to replicate nPM-induced transcriptional changes with additional biologically relevant time points for brain development.
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| Overall design |
Pregrant dams were exposed to nPM during gestational period. The effects were studied in neonate pups.
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| Web link |
https://0-doi-org.brum.beds.ac.uk/10.1186/s11689-021-09380-3
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| Contributor(s) |
Haghani A, Feinberg JI, Lewis KC, Ladd-Acosta C, Johnson RG, Jaffe AE, Sioutas C, Finch CE, Campbell DB, Morgan TE, Volk HE |
| Citation(s) |
34429070 |
| Submission date |
Dec 20, 2019 |
| Last update date |
Aug 25, 2021 |
| Contact name |
Amin Haghani |
| E-mail(s) |
ahaghani@altoslabs.com
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| Organization name |
Altos labs
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| Lab |
Hovath lab
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| Street address |
5510 Morehouse Dr
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| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA597046 |
| SRA |
SRP238413 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142453_gene_counts.csv.gz |
1.0 Mb |
(ftp)(http) |
CSV |
| GSE142453_gene_map.csv.gz |
1.7 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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