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| Status |
Public on Nov 11, 2020 |
| Title |
CDK1-formin signal confers microvillar effacement by an attaching and effacing (A/E) pathogen |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Enterohemorrhagic Escherichia coli (EHEC) induces the attachment and effacement of intestinal microvilli. While the molecular mechanisms contributed to the attachment of EHEC have been thoroughly studied, the underlying mechanisms for the effacement of intestinal microvilli induced by EHEC remained elusive. By focus RNAi screening and genetic analysis in C. elegans and further reconfirmed in human Caco-2 intestinal epithelial cells, we demonstrate that the CDK1-formin signal axis is required for this EHEC-induced microvillar effacement in vitro and in vivo.
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| Overall design |
The mRNA profiles of N2 wild type C. elegans of during pathogen EDL933 and non-pathogen OP50 6 hours treatment were generated by deep sequencing, using Illumina HiSeq 2000.
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| Contributor(s) |
Huang C, Liu B |
| Citation(s) |
33397943 |
| Submission date |
Dec 22, 2019 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Cheng-Rung Huang |
| E-mail(s) |
jackie31428@gmail.com
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| Phone |
886928547266
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| Organization name |
National Cheng Kung University
|
| Street address |
University Road
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| City |
Tainan |
| ZIP/Postal code |
701 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA597224 |
| SRA |
SRP238530 |
