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| Status |
Public on Mar 16, 2009 |
| Title |
Human fibroblast stimulation with PDGF-BB or b-FGF |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We analyzed gene expression in human fibroblasts stimulated by platelet-derived growth factor-BB (PDGF-BB) or basic fibroblast growth factor (bFGF) for 1h and 24h. The results of two independent experiments were merged. SAM analysis identified 116 relevant probe sets. Hierarchical clustering of these probe sets showed divergent early gene regulation by PDGF and FGF but overlapping late response. We first analyzed genes commonly regulated by PDGF-BB and b-FGF more than 2 fold after 24h of stimulation and we found that these two growth factors repressed FOXO.
We then focused on the early gene expression response induced by both growth factors. We performed a fold change analysis and found 114 probe sets regulated by PDGF-BB and 42 probe sets regulated by b-FGF, 37 of which were shared between the two gene lists .
Keywords: Time course, cell Treatment comparison
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| Overall design |
Human foreskin fibroblasts (AG01518) were cultured (1.5E6 cells/10 cm dish) in MEM medium with 10% fetal calf serum and glutamine for 24h, then washed and incubated for 47h in serum-free medium. Cells were stimulated for 1h by PDGF-BB (25ng/ml) or b-FGF (10ng/ml, + heparine 50 microg/ml). Alternatively, cells were incubated for 24h in starvation medium and treated for 24h with growth factors, or left untreated for 48h in serum-free medium (control treatment).
Two biological replicates were performed
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| Contributor(s) |
Essaghir A, Demoulin J |
| Citation(s) |
19244250, 20215436, 24762137 |
| Submission date |
Dec 31, 2008 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Jean-Baptiste Demoulin |
| Organization name |
Université catholique de Louvain
|
| Department |
de Duve Institute
|
| Lab |
UCL - MEXP
|
| Street address |
av Hippocrate 75, B1.74.05
|
| City |
Brussels |
| ZIP/Postal code |
B-1200 |
| Country |
Belgium |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (10)
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| GSM356965 |
PDGF-BB stimulation for 24h_replicate1 |
| GSM356966 |
PDGF-BB stimulation for 1h_replicate1 |
| GSM356967 |
b-FGF stimulation for 24h_replicate1 |
| GSM356968 |
b-FGF stimulation for 1h_replicate1 |
| GSM356969 |
Unstimulated Control_replicate1 |
| GSM356970 |
Unstimulated Control_replicate2 |
| GSM356971 |
b-FGF stimulation for 1h_replicate2 |
| GSM356972 |
b-FGF stimulation for 24h_replicate2 |
| GSM356973 |
PDGF-BB stimulation for 1h_replicate2 |
| GSM356974 |
PDGF-BB stimulation for 24h_replicate2 |
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| Relations |
| BioProject |
PRJNA111211 |
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