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| Status |
Public on Jan 03, 2009 |
| Title |
Transgenerational epigenetic programming of the brain transcriptome and anxiety behavior |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Embryonic exposure to the endocrine disruptor vinclozolin during gonadal sex determination promotes an epigenetic reprogramming of the male germ-line that is associated with transgenerational adult onset disease states. Further analysis of this transgenerational phenotype on the brain demonstrated reproducible changes in the brain transcriptome three generations (F3) removed from the exposure. The transgenerational alterations in the male and female brain transcriptomes were distinct. In the males, the expression of 92 genes in the hippocampus and 276 genes in the amygdala were transgenerationally altered. In the females, the expression of 1,301 genes in the hippocampus and 172 genes in the amygdala were transgenerationally altered. Analysis of specific gene sets demonstrated that several brain signaling pathways were influenced including those involved in axon guidance and long-term potentiation. An investigation of behavior demonstrated that the vinclozolin F3 generation males had a decrease in anxiety-like behavior, while the females had an increase in anxiety-like behavior. These observations demonstrate that an embryonic exposure to an environmental compound appears to promote a reprogramming of brain development that correlates with transgenerational sex-specific alterations in the brain transcriptomes and behavior. Observations are discussed in regards to environmental and transgenerational influences on the etiology of brain disease.
Keywords: expression analysis, transgenerational changes due to vinclozolin
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| Overall design |
Male and female amygdala and hippocampus and male whole brain RNA samples from F3 generation control groups are compared to F3 generation vinclozolin-treated groups.
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| Web link |
http://www.skinner.wsu.edu/
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| Contributor(s) |
Skinner M, Anway M, Savenkova M, Gore AC, Crews D |
| Citation(s) |
19015723 |
| Submission date |
Jan 02, 2009 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Michael K Skinner |
| E-mail(s) |
skinner@mail.wsu.edu
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| Organization name |
WSU
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| Department |
SBS
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| Street address |
Abelson 507
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| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99163 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (20)
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| GSM357074 |
F3-control adult male rat whole brain, replica 1 |
| GSM357075 |
F3-control adult male rat whole brain, replica 2 |
| GSM357076 |
F3-vinclozolin treated adult male rat whole brain, replica 1 |
| GSM357077 |
F3-vinclozolin treated adult male rat whole brain, replica 2 |
| GSM357078 |
F3-control adult male rat amygdala, replica 1 |
| GSM357079 |
F3-control adult male rat amygdala, replica 2 |
| GSM357080 |
F3-vinclozolin treated adult male rat amygdala, replica 1 |
| GSM357081 |
F3-vinclozolin treated adult male rat amygdala, replica 2 |
| GSM357082 |
F3-control adult male rat hippocampus, replica 1 |
| GSM357083 |
F3-control adult male rat hippocampus, replica 2 |
| GSM357084 |
F3-vinclozolin treated adult male rat hippocampus, replica 1 |
| GSM357085 |
F3-vinclozolin treated adult male rat hippocampus, replica 2 |
| GSM357086 |
F3-control adult female rat amygdala, replica 1 |
| GSM357087 |
F3-control adult female rat amygdala, replica 2 |
| GSM357088 |
F3-vinclozolin treated adult female rat amygdala, replica 1 |
| GSM357089 |
F3-vinclozolin treated adult female rat amygdala, replica 2 |
| GSM357090 |
F3-control adult female rat hippocampus, replica 1 |
| GSM357091 |
F3-control adult female rat hippocampus, replica 2 |
| GSM357092 |
F3-vinclozolin treated adult female rat hippocampus, replica 1 |
| GSM357093 |
F3-vinclozolin treated adult female rat hippocampus, replica 2 |
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| Relations |
| BioProject |
PRJNA111233 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14269_RAW.tar |
50.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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