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Series GSE142849 Query DataSets for GSE142849
Status Public on Dec 24, 2021
Title Permanently Re-programmed Microglia in Spinal Cord Injury Contribute to Functional Recovery
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Microglia are resident myeloid cells of the central nervous system (CNS). Recently, single-cell RNA sequencing (scRNAseq) has enabled description of a disease-associated subtype of microglia (DAM) with a role in neurodegeneration and demyelination. In this study we use scRNAseq to investigate the temporal dynamics of immune cells harvested from the epicenter of traumatic spinal cord injury (SCI). As a consequence of SCI, homeostatic microglia undergo permanent transcriptional re-programming into a subtype of microglia with striking similarities to previoysly reported DAM as well as a distinct microglial state found during development. Using a microglia depletion model we showed that DAM in SCI are derived from homeostatic microglia and strongly enhance recovery of hind limb locomotor function following injury.
 
Overall design Investigation of CD45+ immune cells in spinal cord injury (SCI) over time in an experimental mouse model
 
Contributor(s) Hakim R, Zachariadis V, Sankavaram SR, Han J, Harris RA, Brundin L, Enge M, Svensson M
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Submission date Jan 02, 2020
Last update date Dec 25, 2021
Contact name Martin Enge
E-mail(s) martin.enge@ki.se
Organization name Karolinska Institute
Department Dep of Oncology-Pathology
Street address CCK, Z4
City Stockholm
ZIP/Postal code S-171 76
Country Sweden
 
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (3687)
GSM4241227 spinal cord, Naive, VZC00104.A02
GSM4241228 spinal cord, Naive, VZC00104.A03
GSM4241229 spinal cord, Naive, VZC00104.A05
Relations
BioProject PRJNA598659
SRA SRP239303

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142849_RAW.tar 257.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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