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Series GSE14293 Query DataSets for GSE14293
Status Public on Feb 05, 2009
Title Transcriptome analysis of J774A.1 macrophages undergoing amino acid deprivation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Phagocytosis represents a mechanism used by macrophages to remove pathogens and cellular debris. Recent evidence suggested that amino acid or glucose deprivation may cause an increase in phagocytosis of heat-inactivated Escherichia coli and Staphylococcus aureus by macrophages, but not the uptake of platelets, apoptotic cells or beads. Increased phagocytosis of bacteria could be blocked by phagocytosis inhibitors and depended on p38 MAP kinase activity. To examine potentially important downstream pathways linked to EBSS-induced starvation and p38 MAP kinase activation, a full genome microarray representing over 41,000 mouse genes or transcripts was probed with cDNA isolated from J774A.1 macrophages that were treated with EBSS, EBSS supplemented with the p38 inhibitor SB202190 or control medium supplemented with 10% fetal bovine serum.

Keywords: autophagy, heterophagy, p38 MAP kinase, scavenger receptor A, starvation
 
Overall design Prior to RNA isolation, J774A.1 macrophages were incubated in RPMI 1640 medium supplemented with (i) 10% fetal bovine serum, (ii) Earle’s Balanced Salt Solution (EBSS) or (iii) EBSS supplemented with 10 µM SB202190 for 6 hours.
 
Contributor(s) Martinet W, Schrijvers D, Timmermans J, Herman A, De Meyer G
Citation(s) 19302214
Submission date Jan 06, 2009
Last update date Jan 12, 2017
Contact name Rekin's Janky
E-mail(s) Nucleomics.Bioinformatics@vib.be
Organization name VIB
Department Nucleomics Core
Street address Herestraat 49 Box 816
City Leuven
ZIP/Postal code B-3000
Country Belgium
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (3)
GSM357492 251486814895_A01
GSM357493 251486814895_A02
GSM357494 251486814895_A03
Relations
BioProject PRJNA111291

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14293_RAW.tar 42.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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