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| Status |
Public on Jan 20, 2009 |
| Title |
esBAF is an essential component of the core pluripotency transcriptional network |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Distinctive SWI/SNF-like ATP-dependent chromatin remodeling esBAF complexes are indispensable for the maintenance and pluripotency of mouse embryonic stem (ES) cells. To understand the mechanism underlying the roles of these complexes in ES cells, we performed high-resolution genome-wide mapping of the core ATPase subunit, Brg, using ChIP-Seq technology. We find that that esBAF, as represented by Brg, binds to genes encoding components of the core ES transcriptional circuitry, including Polycomb group proteins. esBAF colocalizes extensively with Oct4, Sox2 and Nanog genome-wide, and shows distinct functional interactions with Oct4 and Sox2 at its target genes. Surprisingly, no significant colocalization of esBAF with PRC2 complexes, represented by Suz12, is observed. Lastly, esBAF co-binds with Stat3 and Smad1 genome-wide, consistent with a direct and critical role in LIF and BMP signaling essential to maintain pluripotency. Taken together, our studies indicate that esBAF is both an essential component of the core pluripotency transcriptional network, and might also be a critical component of the LIF and BMP signaling pathways essential for maintenance of self-renewal and pluripotency.
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| Overall design |
Brg knockdown effect on expression, Brg ChIP-Seq
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| Contributor(s) |
Ho L, Jothi R, Ronan J, Cui K, Zhao K, Crabtree GR |
| Citation(s) |
19279218 |
| Submission date |
Jan 08, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Raja Jothi |
| E-mail(s) |
jothi@mail.nih.gov
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| Organization name |
National Institutes of Health
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| Department |
National Institute of Environmental Health Sciences
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| Lab |
Systems Biology
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| Street address |
111 TW Alexander Drive; A314
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| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (8)
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| Relations |
| SRA |
SRP000715 |
| BioProject |
PRJNA111529 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14344_RAW.tar |
255.4 Mb |
(http)(custom) |
TAR (of BED, CEL, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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