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| Status |
Public on Jan 14, 2020 |
| Title |
Caenorhabditis elegans ADAR editing and the ERI-6/7/MOV10 RNAi pathway silence endogenous viral elements and LTR retrotransposons |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
To examine the roles of the ADAR genes and the ERI-6/7 helicase in endogenous RNAi pathways, we sequenced small RNA of adr-1; adr-2; eri-6 mutants. We also analyzed the transcriptomes of these mutants using total RNAseq and mRNAseq. mRNAseq was done in mutants depleted for the RNAi factors drh-1 and nrde-3, or control RNAi
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| |
|
| Overall design |
small RNA, total RNA and mRNA sequencing was done on day 1 adult C. elegans , wild type and mutant or depleted using RNAi
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| |
|
| Contributor(s) |
Fischer SE, Ruvkun G |
| Citation(s) |
32123111 |
| Submission date |
Jan 13, 2020 |
| Last update date |
Mar 30, 2020 |
| Contact name |
Sylvia Fischer |
| E-mail(s) |
sylvia.fischer@childrens.harvard.edu
|
| Organization name |
Boston Children's Hospital
|
| Department |
Division of Infectious Diseases
|
| Street address |
300 Longwood Avenue
|
| City |
Boston |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
|
| Samples (22)
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| Relations |
| BioProject |
PRJNA600993 |
| SRA |
SRP241823 |
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