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| Status |
Public on Nov 08, 2020 |
| Title |
Gene expression profiles of WT and CREPT deleted Lgr5+ intestinal stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting gene, is preferably expressed in the crypts, where the ISCs reside, but not in the villi. The Lgr5+ ISCs have much higher CREPT protein level than Lgr5- cells. To explore the function of CREPT in ISCs, we isolated WT and CREPT deleted Lgr5+ ISCs (Lgr5-CREPTKO) to perform Next generation sequencing.
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| Overall design |
To verify the role of CREPT in Lgr5+ ISCs, we knock out CREPT in Lgr5-GFP+ cells by intraperitoneal injection of Tamoxifen into mice, and sorted the Lgr5-GFPhigh cells from disaggregated WT and Lgr5-CREPTKO intestines. Total RNAs were extracted from sorted cells, and sequenced by BGISEQ-500.
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| Contributor(s) |
Yang L |
| Citation(s) |
33431892 |
| Submission date |
Jan 14, 2020 |
| Last update date |
Jan 26, 2021 |
| Contact name |
Liu Yang |
| E-mail(s) |
yalaceline@hotmail.com
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| Organization name |
Tsinghua University
|
| Street address |
Qinghuayuan street
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| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA601091 |
| SRA |
SRP241886 |
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