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| Status |
Public on Jan 15, 2020 |
| Title |
RNA-sequencing of mouse intestinal stem cells (ISCs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We previously demonstrated that SRCAP regulates the self-renewal of ESCs and modulates lymphoid lineage commitment. We further validated that SRCAP was mainly distributed in liver, spleen and intestine by Northern blot. SRCAP was also highly expressed in Lgr5+ ISCs. We then sought to explore the physiological role of SRCAP in the regulation of self-renewal maintenance of ISCs.We then generated Srcapflox/flox mice through insertion of loxP sequences flanking at the exon5 of Srcap gene locus. We established Srcapflox/flox;Lgr5GFP-CreERT2 mice through crossing Srcapflox/flox mice with Lgr5GFP-CreERT2 mice. With administration of tamoxifen (TAM), Srcap was completely deleted in Lgr5+ ISCs. We identified that Srcap deficiency impairs the self-renewal of ISCs and intestinal epithelial regeneration. Through RNA-sequencing, we sough to identify the key gene regulated by SRCAP in ISC self-renewal.
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| Overall design |
Total RNAs were isolated from WT or SRCAP-deficient ISCs and used for RNA sequencing
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| Contributor(s) |
Ye B, Liu B |
| Citation missing |
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| Submission date |
Jan 14, 2020 |
| Last update date |
Jan 18, 2020 |
| Contact name |
Benyu Liu |
| E-mail(s) |
benyuliu2014@163.com
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| Organization name |
Institute of Biophysics, CAS
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| Department |
The CAS key Laboratory of Infection and Immunity, Institute of Biophysics
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| Lab |
Zusen Fan
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| Street address |
15, Datun Road, Chaoyang district
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA601195 |
| SRA |
SRP241933 |
