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Series GSE143695 Query DataSets for GSE143695
Status Public on Nov 08, 2020
Title Gene expression profiles of WT and Vil-CREPTKO intestinal cyrpts
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting gene, is preferably expressed in the crypts, where the ISCs reside, but not in the villi. To explore the function of CREPT in ISCs, we isolated crypts to perform Next generation sequencing.
 
Overall design To verify the role of CREPT in intestine, we knock out CREPT in intestinal epithelium by introducing villin-cre into CREPT flox/flox mice. Crypts were disaggregated from intestinal epithelium of WT and Vil-CREPTKO mice. Total RNAs were extracted from crypts, and sequenced by Illumina XTEN
 
Contributor(s) Yang L
Citation(s) 33431892
Submission date Jan 15, 2020
Last update date Jan 26, 2021
Contact name Liu Yang
E-mail(s) yalaceline@hotmail.com
Organization name Tsinghua University
Street address Qinghuayuan street
City Beijing
ZIP/Postal code 100084
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM4272742 Vil_WT_1
GSM4272743 Vil_WT_2
GSM4272744 Vil_KO_1
Relations
BioProject PRJNA601321
SRA SRP242012

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143695_Vil_WT_KO_readcount.txt.gz 386.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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