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Status |
Public on Nov 08, 2020 |
Title |
Gene expression profiles of WT and Vil-CREPTKO intestinal cyrpts |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting gene, is preferably expressed in the crypts, where the ISCs reside, but not in the villi. To explore the function of CREPT in ISCs, we isolated crypts to perform Next generation sequencing.
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Overall design |
To verify the role of CREPT in intestine, we knock out CREPT in intestinal epithelium by introducing villin-cre into CREPT flox/flox mice. Crypts were disaggregated from intestinal epithelium of WT and Vil-CREPTKO mice. Total RNAs were extracted from crypts, and sequenced by Illumina XTEN
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Contributor(s) |
Yang L |
Citation(s) |
33431892 |
Submission date |
Jan 15, 2020 |
Last update date |
Jan 26, 2021 |
Contact name |
Liu Yang |
E-mail(s) |
yalaceline@hotmail.com
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Organization name |
Tsinghua University
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Street address |
Qinghuayuan street
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City |
Beijing |
ZIP/Postal code |
100084 |
Country |
China |
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Platforms (1) |
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Samples (4)
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Relations |
BioProject |
PRJNA601321 |
SRA |
SRP242012 |