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Series GSE143740 Query DataSets for GSE143740
Status Public on Oct 06, 2021
Title miRNA expression profiling and identification of a miRNA regulator for macrophage polarization in the murine alveolar macrophage cell line MH-S.
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Using the highly sensitive miRNA array, we screened differentially regulated miRNAs in IL-4-induced M2 macrophages and explored the functions of miR-378a-3p by Gene Ontology and Kyoto Encyclopedia of Genes annotation. Furthermore, the quantitative real-time polymerase chain reaction, western blot and dual luciferase reporter assay results showed that Grb2 is a specific target of miR-378a-3p, which will give new insights in disclosing the mechanism of macrophage polarization.
Overall design In this study, IL-4 induced miRNAs expression in murine alveolar macrophage cell line was measured at 48 hours after exposure to 20 ng/mL IL-4. Three independent experiments were performed.
Contributor(s) Wang Q, Jiang S
Citation(s) 34589519
Submission date Jan 15, 2020
Last update date Oct 07, 2021
Contact name Qiujie Wang
Organization name Sun Yat-sen University Second University Hospital
Department Department of Respiratory Medicine
Street address No.107 Yan jiang West Road
City Guangzhou
State/province Guangdong
ZIP/Postal code 510120
Country China
Platforms (1)
GPL18058 Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18, condensed Probe_ID version]
Samples (6)
GSM4273520 MH-S_control rep1
GSM4273521 MH-S_control rep2
GSM4273522 MH-S_control rep3
BioProject PRJNA601470

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143740_RAW.tar 5.6 Mb (http)(custom) TAR (of GPR)
Raw data provided as supplementary file
Processed data included within Sample table

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