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| Status |
Public on Sep 16, 2021 |
| Title |
Transcriptional profiling of distinct fibroblast populations in the pancreatic tumor microenvironment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pancreatic stellate cells are thought to be the predominant source of cancer-associated fibroblasts (CAFs) in pancreatic cancer. We developed a mouse model which allows us to track and analyze stellate cells and stellate cell-derived CAFs in vivo during pancreatic tumorigenesis for the first time. We find that stellate cells in fact give rise to a minority of all CAFs. Here, we have used lineage reporters to isolate stellate cell-derived and non-stellate cell-derived CAFs and compared them by RNA-seq.
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| Overall design |
Rosa-mTmG; Fabp4-Cre mice at 8 weeks of age were injected with FC1199 murine pancreatic cancer cells orthotopically. After 4 weeks, tumors were harvested, single cell suspensions generated, and FACS performed to isolate GFP+ (stellate cell-derived) and Tomato+ (non-stellate cell-derived) CAFs. RNA was isolated and RNA-seq performed on both cell populations.
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| Contributor(s) |
Sherman M, Horton W, Helms E |
| Citation(s) |
34548310 |
| Submission date |
Jan 16, 2020 |
| Last update date |
Dec 16, 2021 |
| Contact name |
Mara Sherman |
| E-mail(s) |
shermama@ohsu.edu
|
| Organization name |
Oregon Health & Science University
|
| Street address |
2730 SW Moody Avenue CL6C
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97201 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA601743 |
| SRA |
SRP242232 |
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