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| Status |
Public on Dec 31, 2009 |
| Title |
Ovarian Cancer gene expression profiling identifies the surface of the ovary as a stem cell niche |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In contrast to epithelial derived carcinomas that arise in most human organs, ovarian surface epithelial cells become more rather than less differentiated as the malignancy progresses. To test the hypothesis that ovarian surface epithelial cells retain properties of relatively uncommitted pluripotent cells until undergoing neoplastic transformation, we conducted gene expression profiling analysis (Affymetrix, U133 Plus 2.0) of 12 ovarian surface epithelial cells and 12 laser capture microdissected serous papillary ovarian cances. We find that over 2000 genes are significantly differentially expressed between the surface epithelial and cancer samples. Network analysis implicates key signaling pathways and pathway interactions in ovarian cancer development. Genes previously associated with adult stem cell maintenance are expressed in ovarian surface epithelial cells and significantly down-regulated in ovarian cancer cells. Our results indicate that the surface of the ovary is an adult stem cell niche and that deregulation of genes involved in maintaining the quiescence of ovarian surface epithelial cells is instrumental in the initiation and development of ovarian cancer.
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| Overall design |
Twelve healthy ovarian surface epithelia samples (OSE) were compared to twelve laser capture microdissected serous ovarian cancer epithelia samples (CEPI) via Affymetrix 3' expression array.
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| Contributor(s) |
Bowen NJ, Walker LD, Matyunina LV, Totten K, Benigno BB, McDonald JF |
| Citation(s) |
20040092 |
| Submission date |
Jan 13, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Nathan J. Bowen |
| E-mail(s) |
nbowen@cau.edu
|
| Phone |
4048808158
|
| Organization name |
Clark Atlanta University
|
| Department |
Center for Cancer Research and Therapeutic Development
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| Lab |
Cancer Development and Evolution
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| Street address |
223 James P Brawley Drive
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30314 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA111799 |
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