|
| Status |
Public on Jan 24, 2020 |
| Title |
Peri-infarct region transcriptome of SCID mice trated with saline control, iPSC-derived cardiomyocytes (iCM), or iCM-derived exosomes (iCM-Ex) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
iPSC-derived iCMs represent a potential therapy for ischemic cardiomyopathy. Previous studies demonstrated enhanced cardiac function following injection of iCMs into the peri-infarct region in mice models. Cardioprotection despite poor engraftment suggests that iCMs may modulate their therapeutic activity by secreted molecules. Exosomes are discrete packages of secreted molecules that are readily taken up by target cells. iCM and iCM-Ex treatment of acute myocardial infarctions (Mis) significantly improved cardiac function in SCID mice.
We used microarrays to identify differentially-regulated genes in iCM- or iCM-Ex treated mice and control mice.
|
| |
|
| Overall design |
Female 80-100 day SCID mice received acute MIs by LAD ligation and injections of iCMs, iCM-Ex, or saline. Four weeks post-MI, mice were sacrificed and the hearts were explanted. We focused our investigation on the peri-infarct region (PIR) and removed the core infarct and remote zones.
|
| |
|
| Contributor(s) |
Santoso MR, Yang PC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 23, 2020 |
| Last update date |
Jan 26, 2020 |
| Contact name |
Phillip Yang |
| Organization name |
Stanford University School of Medicine
|
| Street address |
269 Campus Drive, CCSR 3120
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
|
| Samples (9)
|
| GSM4282397 |
PIR from mouse injected with saline control, biological rep1 |
| GSM4282398 |
PIR from mouse injected with saline control, biological rep2 |
| GSM4282399 |
PIR from mouse injected with saline control, biological rep3 |
| GSM4282400 |
PIR from mouse injected with iCMs, biological rep1 |
| GSM4282401 |
PIR from mouse injected with iCMs, biological rep2 |
| GSM4282402 |
PIR from mouse injected with iCMs, biological rep3 |
| GSM4282404 |
PIR from mouse injected with iCM-Ex, biological rep1 |
| GSM4282406 |
PIR from mouse injected with iCM-Ex, biological rep2 |
| GSM4282407 |
PIR from mouse injected with iCM-Ex, biological rep3 |
|
| Relations |
| BioProject |
PRJNA602924 |
Less...
More...