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| Status |
Public on Dec 30, 2011 |
| Title |
Change in expression of genes after retinoic acid treatment of stellate cells: time course |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We evaluated the change in expression of genes after treatment of stellate cells with retinoic acid to understand how the stellate cells can de-differentiate and effect their physiological behaviour in pathological conditions. We then tested the changes in the gene expression in 2D and 3D culture conditions for pancreatic stellate cells and in a pancreatic cancer model.
Keywords: gene expression change, time course
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| Overall design |
We treated a pancreatic stellate cell line on plastic with 1 micromolar all-trans retinoic acid (ATRA) for timepoints of 30 mins, 4 hours, 12 hours, 24 hours and 168 hours. RNA was extracted and hybridized on Illumina Human microarrays. We looked for target genes regulated by ATRA and evaluated for time course change.
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| Contributor(s) |
Kocher HM, Chelala C, Froeling FE, Dobson R, Mein CE, Hart IR |
| Citation(s) |
21704588 |
| Submission date |
Jan 14, 2009 |
| Last update date |
Feb 15, 2013 |
| Contact name |
Hemant Kocher |
| E-mail(s) |
h.kocher@qmul.ac.uk
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| Organization name |
Barts and the London
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| Street address |
Charterhouse Square
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| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6102 |
Illumina human-6 v2.0 expression beadchip |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE14427 |
Change in expression of genes after retinoic acid treatment of stellate cells |
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| Relations |
| BioProject |
PRJNA114495 |
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