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Series GSE14429 Query DataSets for GSE14429
Status Public on Jan 15, 2009
Title Alteration of gene expression profile in HEK293 cells treated with proteasome inhibitor epoxomicin
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The objective of this study is to identify the genes that are up-regulated amid proteasome dysfunction to facilitate the discovery of proteolytic pathways that are activated as a compensatory response to proteasome inhibition. Proteasome is a large multi-component proteolytic complex in the cell. It is responsible for the constitutive turn-over of many cellular proteins as well as the degradation of oxidized and/or unfolded proteins. With such a fundamental role in the cell, disruption of proteasome understandably can lead to disastrous outcome. Oxidative stress has been postulated as the driving mechanism for aging. Oxidatively modified proteins, which usually have lost their activity, require immediate removal by proteasome to maintain normal cellular function. Dysfunction of proteasome has also been linked to neuro-degenerative diseases such as Alzheimer’s and Parkinson’s diseases, those that are most commonly seen in aged population. There is more than one proteolytic pathway in the cell, and it has been reported that obstruction of any one of these pathways may enhance the activity of the others. Proteasomal function has been found to have decreased during aging, prompting researchers to hypothesize that failure to remove oxidized proteins may play an important role in aging. It would be interesting to determine the other proteolytic pathways that are activated after proteasome inhibition by a relatively specific inhibitor epoxomicin to help understand their roles in aging processes.

Keywords: time course, proteasome, inhibitor, oxidative stress, epoxomicin
Overall design Human embryonic kidney cells (HEK293) were untreated or treated with 1 uM epoxomicin for 1 and 6 hours. Total RNA was extracted for gene expression profiling using Affymetrix human genome U133 2.0 plus chip. A quadrupliate was prepared for each treatment.
contributor: NHLBI Gene Expression Core Facility
Contributor(s) Chang AH, Levine RL
Citation(s) 21558272
Submission date Jan 14, 2009
Last update date Mar 25, 2019
Contact name Allen HK Chang
Organization name NIH\NHLBI
Lab Laboratory of Biochemistry
Street address 50 South Drive, Bldg.50, Rm.2347
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (12)
GSM360320 Human embryonic kidney cells (HEK293) at 0hr, replicate 1
GSM360321 Human embryonic kidney cells (HEK293) at 0hr, replicate 2
GSM360322 Human embryonic kidney cells (HEK293) at 0hr, replicate 3
BioProject PRJNA111757

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Supplementary file Size Download File type/resource
GSE14429_RAW.tar 52.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Raw data provided as supplementary file

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