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| Status |
Public on Apr 15, 2009 |
| Title |
Gene expression profiles of spontaneous metastasis in a K-ras/p53 mutant mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The biologic basis for NSCLC metastasis is not well understood. Here we addressed this deficiency by transcriptionally profiling tumors from a genetic mouse model of human lung adenocarcinoma that develops metastatic disease owing to the expression of K-rasG12D and p53R172H. We identified 2,209 genes that were differentially expressed in distant metastases relative to matched lung tumors. Mining of publicly available data bases revealed this expression signature in a subset of NSCLC patients who had a poorer prognosis than those without the signature.
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| Overall design |
Primary lung adenocarcinomas and metastases from p53R172H∆g/+ K-rasLA1/+ mice or syngeneic tumors were isolated, carefully dissected to remove the adjacent tissue, snap-frozen in liquid nitrogen and stored at -80° until use. Part of each dissected tumor was histologically evaluated by a board-certified pathologist. Synthesis of cRNA and hybridization to Mouse Expression Array 430A 2.0 chips were performed. Two-sided t-paired tests using log-transformed expression values determined significant differences between primary tumors and metastasis.
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| Contributor(s) |
Creighton C, Kurie J, Berel D, Gibbons D, Lin W |
| Citation(s) |
19404390 |
| Submission date |
Jan 15, 2009 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
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| Organization name |
Baylor College of Medicine
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| Department |
Biostatistics, Ducan Cancer Center
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| Street address |
One Baylor Plaza, Mail Stop: BCM305
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (16)
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| GSM361017 |
Mouse 4, Sample:SZ32, Sex:M, Metastasis, Mediastinal LN |
| GSM361018 |
Mouse 1, Sample:SZ20, Sex:M, Metastasis, s.c. met/body wall |
| GSM361019 |
Mouse 3, Sample:SZ26, Sex:M, Metastasis, Mediastinal LN |
| GSM361020 |
Mouse 4, Sample:SZ33, Sex:M, Metastasis, Parietal pleural nodule |
| GSM361021 |
Mouse 3, Sample:SZ27, Sex:M, Metastasis, s.c. met |
| GSM361022 |
Mouse 1, Sample:SZ19, Sex:M, Metastasis, Liver met |
| GSM361023 |
Mouse 1, Sample:SZ21, Sex:M, Metastasis, Inguinal met |
| GSM361024 |
Mouse 2, Sample:SZ23, Sex:M, Metastasis, Liver met |
| GSM361025 |
Mouse 3, Sample:SZ25, Sex:M, Metastasis, heart met |
| GSM361026 |
Mouse 5, Sample:SZ14, Sex:M, Primary lung cancer, Adenocarcinoma |
| GSM361027 |
Mouse 6, Sample:SZ15, Sex:F, Primary lung cancer, Adenocarcinoma |
| GSM361028 |
Mouse 7, Sample:SZ17, Sex:M, Primary lung cancer, Adenocarcinoma |
| GSM361029 |
Mouse 2, Sample:SZ22, Sex:M, Primary lung cancer, Adenocarcinoma |
| GSM361030 |
Mouse 4, Sample:SZ31, Sex:M, Primary lung cancer, Adenocarcinoma |
| GSM361031 |
Mouse 1, Sample:SZ18, Sex:M, Primary lung cancer, Adenocarcinoma |
| GSM361032 |
Mouse 3, Sample:SZ24, Sex:M, Primary lung cancer, Adenocarcinoma |
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| This SubSeries is part of SuperSeries: |
| GSE14459 |
NSCLC metastasis: K-ras/p53 mutant and syngeneic mouse models |
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| Relations |
| BioProject |
PRJNA114501 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14449_RAW.tar |
34.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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