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| Status |
Public on May 31, 2009 |
| Title |
Quiescent keratinocytes and fibroblasts: radiation dose response |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Skin is usually exposed during human exposures to ionizing radiation, however there are few experiments that evaluate the radiation responsiveness of the cells of the epidermis (keratinocytes) and those of the dermis (fibroblasts) in the same studies. We evaluated the transcriptional responses of quiesent primary keratinocytes and fibroblasts from the same individual and compared them with quiescent keratinocytes and fibroblasts that were immortalized by human telomerase (hTert).
The primary transcriptional responses to 10-500 cGy ionizing radiation were p53-mediated responses; however, we did identify distinct responses between the keratinocytes and the fibroblasts.
Keywords: keratinocytes and fibroblasts - dose response to ionizing radiation
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| Overall design |
Four cell types (primary keratinocytes, hTert immortalized keratinocytes, primary fibroblasts, hTert immortalized fibroblasts) grown to quiescence, treated with 0, 10, 100 or 500 cGy gamma irradiation, RNA collected at 4 hrs.
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| |
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| Contributor(s) |
Warters RL, Moos PJ |
| Citation(s) |
19580510 |
| Submission date |
Jan 16, 2009 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Philip J Moos |
| E-mail(s) |
philip.moos@utah.edu
|
| Phone |
801-585-5952
|
| Organization name |
University of Utah
|
| Department |
Pharmacology & Toxicology
|
| Lab |
Moos
|
| Street address |
30 S 2000 East, Rm 201
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1708 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA111465 |
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