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| Status |
Public on Aug 20, 2020 |
| Title |
poly A+ RNA sequencing of quiescent and early serum stimulated WI-38s |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed paired-end poly A+ RNA sequencing using total RNA isolated from serum starved and serum released WI-38 cells
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| |
|
| Overall design |
Quiescent WI-38s were induced by serum starvation (0.1% FBS) for 3 days, and collected for RNA extraction. Serum stimulation was performed in quiescent cells by changing back to 10% FBS contained medium. Cells at early serum release time points at 3 hour and 6 hour were collected for RNA extraction.
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| |
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| Contributor(s) |
Sun Q, Prasanth KV |
| Citation(s) |
32675111 |
| Submission date |
Feb 03, 2020 |
| Last update date |
Aug 22, 2020 |
| Contact name |
Prasanth Kumar Vijayan Kannanganattu |
| E-mail(s) |
kumarp@illinois.edu
|
| Phone |
2173512025
|
| Organization name |
University of Illinois at Urbana-Champaign
|
| Department |
Cell and Developmental Biology
|
| Lab |
Prasanth lab
|
| Street address |
601S, Goodwin Avenue
|
| City |
Urbana |
| State/province |
Illinois |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA604619 |
| SRA |
SRP246951 |
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