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| Status |
Public on Jan 29, 2021 |
| Title |
Genome-wide maps of QKI-5, SREBP2, and POL II in eye lens cells |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We found the genome-wide co-binding of QKI-5 and SREBP2. Notably, the genomic distribution analysis revealed that the co-binding events between QKI-5 and SREBP2 highly occurred at the regions of the promoter/transcription start site (TSS), where active transcription was taken place in a lens cell-specific manner, which was indicated by POL II binding events. Cellular pathway analysis of the genes whose promoters were co-bound by QKI-5, SREBP2, and POL II showed a significant enrichment of the SREBP2-medaited cholesterol biosynthesis pathway, and QKI depletion led to reduced co-occupancies of SREBP2 and POL II on the promoters of the genes involved in cholesterol biosynthesis. These data suggest QKI-5 as a potential transcription co-activator mediating SREBP2-dependent cholesterol biosynthesis in eye lens cells.
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| Overall design |
We performed ChIP-seq of QKI-5, SREBP2 and Pol II in human lens epithelial (HLE-B3) cells and neural stem cell-derived lens progenitor-like cells of WT and QKI KO.
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| Contributor(s) |
Shin S, He C, Hu J, Lan F |
| Citation(s) |
34021134 |
| Submission date |
Feb 04, 2020 |
| Last update date |
May 23, 2021 |
| Contact name |
Chenxi He |
| E-mail(s) |
cxhe15@fudan.edu.cn
|
| Organization name |
Fudan University
|
| Street address |
131 Dong'an Rd, Mingdao Bldg, Rm 512
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (4)
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| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA604795 |
| SRA |
SRP247176 |
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