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Series GSE14493 Query DataSets for GSE14493
Status Public on Oct 12, 2009
Title Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity during hypoxia in Arabidopsis root tips
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Plant organs are comprised of distinct cell types with unique assemblages of mRNAs. This is a collection of CEL files of mRNA profiles of the total steady-state mRNAs and polysomal mRNAs of distinct cell types of the root tip of 7-d-old Arabidopsis thaliana seedlings. The cell type specific mRNA populations are those present in ribosome-mRNA complexes. This sub-population of mRNAs was obtained by first establishing a collection of Arabidopsis lines that express a FLAG-epitope tagged ribosomal protein L18 (RPL18) directed by promoters expressed in specific cell types and regions. Four different promoter:FLAG-RPL18 lines were used. The targeted cell types and promoters included root endodermis (pSCR) and root stelar xylem and pericycle (pWOL, pSHR). A CaMV 35S promoter:FLAG-RPL18 line was used to obtain the polysomal mRNA of multiple cell types. The immunopurification of ribosome-mRNA complexes of specific cell types was accomplished by the method described in Zanetti et al. (Plant Physiology, 138, 624-635; 2005). Hybridization of the immunopurified mRNAs to the Affymetrix ATH1 DNA microarray platform and subsequent data analysis permitted the identification of transcripts that are enriched or depleted in specific cell types of root tips. The dataset includes samples from cell types from seedlings grown under control conditions and cell types of seedlings exposed to low oxygen stress (hypoxia) for 2 h.

Keywords: cell-type specific expression, hypoxic stress, polysomal mRNA, abiotic stress, endodermis, stele
 
Overall design 20 samples, 2 conditions (2 h hypoxia stress, 2 h non-stress), 2 RNA pools (Total mRNA and polysomal mRNA), 4 promoter lines, 2 replicates
 
Contributor(s) Mustroph A, Bailey-Serres J, Zanetti ME, Holtan HE, Repetti PP
Citation(s) 19843695
Submission date Jan 21, 2009
Last update date Aug 28, 2018
Contact name Angelika Mustroph
E-mail(s) angelika.mustroph@uni-bayreuth.de
Organization name University Bayreuth
Department Plant Physiology
Lab Angelika Mustroph
Street address Universitaetsstr. 30, NWI
City Bayreuth
ZIP/Postal code 95440
Country Germany
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (20)
GSM361976 roottip_control_total_rep1
GSM361977 roottip_control_total_rep2
GSM361978 roottip_hypoxia_total_rep1
This SubSeries is part of SuperSeries:
GSE14578 Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity during hypoxia in Arabidopsis
Relations
Affiliated with GSE69995
BioProject PRJNA114485

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14493_RAW.tar 42.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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