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Status |
Public on Oct 12, 2009 |
Title |
Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity during hypoxia in Arabidopsis root tips |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
Plant organs are comprised of distinct cell types with unique assemblages of mRNAs. This is a collection of CEL files of mRNA profiles of the total steady-state mRNAs and polysomal mRNAs of distinct cell types of the root tip of 7-d-old Arabidopsis thaliana seedlings. The cell type specific mRNA populations are those present in ribosome-mRNA complexes. This sub-population of mRNAs was obtained by first establishing a collection of Arabidopsis lines that express a FLAG-epitope tagged ribosomal protein L18 (RPL18) directed by promoters expressed in specific cell types and regions. Four different promoter:FLAG-RPL18 lines were used. The targeted cell types and promoters included root endodermis (pSCR) and root stelar xylem and pericycle (pWOL, pSHR). A CaMV 35S promoter:FLAG-RPL18 line was used to obtain the polysomal mRNA of multiple cell types. The immunopurification of ribosome-mRNA complexes of specific cell types was accomplished by the method described in Zanetti et al. (Plant Physiology, 138, 624-635; 2005). Hybridization of the immunopurified mRNAs to the Affymetrix ATH1 DNA microarray platform and subsequent data analysis permitted the identification of transcripts that are enriched or depleted in specific cell types of root tips. The dataset includes samples from cell types from seedlings grown under control conditions and cell types of seedlings exposed to low oxygen stress (hypoxia) for 2 h.
Keywords: cell-type specific expression, hypoxic stress, polysomal mRNA, abiotic stress, endodermis, stele
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Overall design |
20 samples, 2 conditions (2 h hypoxia stress, 2 h non-stress), 2 RNA pools (Total mRNA and polysomal mRNA), 4 promoter lines, 2 replicates
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Contributor(s) |
Mustroph A, Bailey-Serres J, Zanetti ME, Holtan HE, Repetti PP |
Citation(s) |
19843695 |
Submission date |
Jan 21, 2009 |
Last update date |
Aug 28, 2018 |
Contact name |
Angelika Mustroph |
E-mail(s) |
angelika.mustroph@uni-bayreuth.de
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Organization name |
University Bayreuth
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Department |
Plant Physiology
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Lab |
Angelika Mustroph
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Street address |
Universitaetsstr. 30, NWI
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City |
Bayreuth |
ZIP/Postal code |
95440 |
Country |
Germany |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (20)
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This SubSeries is part of SuperSeries: |
GSE14578 |
Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity during hypoxia in Arabidopsis |
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Relations |
Affiliated with |
GSE69995 |
BioProject |
PRJNA114485 |