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Series GSE14495 Query DataSets for GSE14495
Status Public on Feb 04, 2009
Title Gene profiling of Müller glia during early stages of zebrafish photoreceptor regeneration
Organism Danio rerio
Experiment type Expression profiling by array
Summary Photoreceptor damage in adult mammals results in permanent cell loss and glial scarring in the retina. In contrast, adult zebrafish can regenerate photoreceptors following injury. By using a stable transgenic line in which GFP is driven by the cis-regulatory sequences of a glial specific marker gfap, Tg(gfap:GFP)mi2002, previous studies showed that Müller glia, the radial glial cells in the retina, proliferate after photoreceptor loss and give rise to neuronal progenitors that eventually differentiate into regenerated photoreceptors. To identify the molecular mechanisms that initiate this regenerative response, Müller glia were isolated from Tg(gfap:GFP)mi2002 fish during the early stages of regeneration after light lesion and gene expression profiles were generated by microarray analyses.

Keywords: time course
Overall design Retinas were dissected from Tg(gfap:GFP)mi2002 zebrafish at 8, 16, 24, 36 hour post-lesion (hpl) and non-light-treated controls (0 hpl) and were dissociated by enzymatic digestion. GFP+ Müller glia were isolated by fluorescence-activated cell sorting (FACS) for RNA extraction and hybridization on Affymetrix microarrays. Independent hybridization of three biological replicates were performed for each time point.
Contributor(s) Qin Z, Brooks M, Khanna R, Raymond PA
Citation(s) 19474300
Submission date Jan 21, 2009
Last update date Jan 25, 2018
Contact name Zhao Qin
Phone 734-764-7476
Organization name University of Michigan
Department Molecular, Cellular and Developmental Biology
Street address 830 North University
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (15)
GSM362009 Müller glia at 0 hpl, biological rep1
GSM362010 Müller glia at 0 hpl, biological rep2
GSM362011 Müller glia at 0 hpl, biological rep3
BioProject PRJNA111551

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14495_RAW.tar 29.2 Mb (http)(custom) TAR (of CEL)
GSE14495_complete_analysis.txt 704.6 Kb (ftp)(http) TXT
GSE14495_readme.txt 98 b (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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