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| Status |
Public on Aug 01, 2020 |
| Title |
Two novel PLS-class pentatricopeptide repeat proteins are involved in the group II intron splicing of mitochondrial transcripts in the moss Physcomitrella patens |
| Organism |
Physcomitrium patens |
| Experiment type |
Expression profiling by array
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| Summary |
Pentatricopeptide repeat (PPR) proteins are RNA binding proteins that function in posttranscriptional regulation as gene-specific regulators of RNA metabolism in plant organelles. Plant PPR proteins are divided into four classes: P, PLS, E and DYW. The E- and DYW-class proteins are mainly implicated in RNA editing, whereas most of the P-class proteins predominantly participate in RNA cleavage, splicing and stabilization. In contrast, the functions of PLS-class proteins still remain obscure. Here, we report the function of PLS-class PpPPR_31 and PpPPR_9 in Physcomitrella patens. The knockout (KO) mutants of PpPPR_31 and PpPPR_9 exhibited slower protonema growth compared to the wild type. The PpPPR_31 KO mutants showed a considerable reduction in the splicing of nad5 intron 3 and atp9 intron 1. The PpPPR_9 KO mutants displayed severely reduced splicing of cox1 intron 3. An RNA electrophoresis mobility shift assay showed that the recombinant PpPPR_31 protein bound to the 5' region of nad5 exon 4 and the bulged-A region in domain VI of atp9 group II intron 1 while the recombinant PpPPR_9 bound to the translated region of ORF622 in cox1 intron 3. These results suggest that PLS-class PPR proteins may influence the splicing efficiency of mitochondrial group II introns.
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| Overall design |
To characterize the function of a mitochondria-localized P-class PPR protein PpPPR_31 in Physcomitrella patens, gene expression of organellar genome of 4 day old protonema cells of PpPPR_31 KO mutants and wild type were measured.
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| Contributor(s) |
Ichinose M, Ishimaru A, Nakajima K, Sugita C, Kawaguchi Y, Sugita M |
| Citation missing |
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| Submission date |
Feb 10, 2020 |
| Last update date |
Aug 03, 2020 |
| Contact name |
Chieko Sugita |
| E-mail(s) |
chie@gene.nagoya-u.ac.jp
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| Organization name |
Nagoya University
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| Department |
Center for Gene Research
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| Lab |
Sugita lab
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| Street address |
Chikusa-ku Furo-cho
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| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-8602 |
| Country |
Japan |
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| Platforms (1) |
| GPL25703 |
Agilent-084494 Physcomitrella patens custom microarray (Ppatens-organella3) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA605799 |
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