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| Status |
Public on Oct 19, 2021 |
| Title |
Neuronal HSF-1 activity coordinates fat desaturation across tissues in C. elegans via TGF-b/BMP signalling |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process that has been previously described to be cell-autonomous. We have discovered that, in Caenorhabditis elegans, the neuronal over-expression of the master regulator of the conserved heat shock response (HSR), Hsf-1, causes extensive fat remodelling in peripheral tissues. These changes include a decrease in fat desaturase expression in the gut, and a shift in the saturation levels of fatty acids in the plasma membrane, in line with ectothermic adaptive responses. We have identified the cGMP receptor TAX-2/TAX-4 and TGF-β/BMP signalling, as key players in signalling across tissues. We also find that gradual increments in ambient temperature result in HSR activation exclusively in wild-type head neurons. This is the first study to suggest that a thermostat-based mechanism can non-cell autonomously coordinate membrane fluidity in response to warm temperatures across tissues in multicellular animals.
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| Overall design |
RNA-seq comparison between young adult N2 (WT) and AGD1289 (neuronal over-expression of HSF-1)
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| Contributor(s) |
Chauve L, Murdoch S, Masoudzadeh F, Hodge F, Lopez-Clavijo A, Okkenhaug H, West G, Segonds-Pichon A, Wingett SW, Kienberger H, Wakelam M, Casanueva O |
| Citation(s) |
34723964 |
| Submission date |
Feb 11, 2020 |
| Last update date |
Nov 16, 2021 |
| Contact name |
Steven William Wingett |
| E-mail(s) |
steven.wingett@mrc-lmb.cam.ac.uk
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| Organization name |
MRC Laboratory of Molecular Biology
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| Department |
Cell Biology
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| Street address |
Francis Crick Avenue, Cambridge Biomedical Campus
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| City |
Cambridge |
| State/province |
Cambs |
| ZIP/Postal code |
CB2 0QH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA606076 |
| SRA |
SRP248277 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145123_DEseq_analysis_N2_AGD1289.xlsx |
3.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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